Mouse/Rat Serpin F1/PEDF Antibody Summary
Asp43-Thr417
Accession # BAA31978
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
Detection of Mouse and Rat Serpin F1/PEDF by Western Blot. Western blot shows lysates of C2C12 mouse myoblast cell line, NIH-3T3 mouse embryonic fibroblast cell line, 3T3-L1 mouse embryonic fibroblast adipose-like cell line, NRK rat normal kidney cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Mouse/Rat Serpin F1/PEDF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1149) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for Serpin F1/PEDF at approximately 46-48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Mouse Serpin F1/PEDF by Simple WesternTM. Simple Western lane view shows lysates of NIH-3T3 mouse embryonic fibroblast cell line, loaded at 0.2 mg/mL. A specific band was detected for Serpin F1/PEDF at approximately 55 kDa (as indicated) using 20 µg/mL of Goat Anti-Mouse/Rat Serpin F1/PEDF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1149) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Serpin F1/PEDF
Serpin F1 is a secreted glycoprotein that shares sequence and structural similarity with the serpin (serine proteinase inhibitor) superfamily proteins but lacks serine protease inhibitory activity.
Product Datasheets
Citations for Mouse/Rat Serpin F1/PEDF Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 6
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Pigment Epithelium-Derived Factor Released by Muller Glial Cells Exerts Neuroprotective Effects on Retinal Ganglion Cells
Authors: Unterlauft JD, Eichler W, Kuhne K et al.
Neurochem Res
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Characterization of the Skeletal Muscle Proteome in Undernourished Old Rats
Authors: C Barbé, J Salles, C Chambon, C Giraudet, P Sanchez, V Patrac, P Denis, Y Boirie, S Walrand, M Gueugneau
International Journal of Molecular Sciences, 2022-04-26;23(9):.
Species: Rat
Sample Types: Tissue Homogenates
Applications: Western Blot -
Exercise plasma boosts memory and dampens brain inflammation via clusterin
Authors: De Miguel Z, Khoury N, Betley MJ et al.
Nature
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Systemic administration of erythropoietin inhibits retinopathy in RCS rats.
Authors: Shen W, Chung S, Irhimeh M, Li S, Lee S, Gillies M
PLoS ONE, 2014-08-13;9(8):e104759.
Species: Rat
Sample Types: Tissue Homogenates
Applications: Western Blot -
Adeno-associated virus vector-mediated delivery of pigment epithelium-derived factor restricts neuroblastoma angiogenesis and growth.
Authors: Streck CJ, Zhang Y, Zhou J, Ng C, Nathwani AC, Davidoff AM
J. Pediatr. Surg., 2005-01-01;40(1):236-43.
Species: Mouse
Sample Types: Serum
Applications: ELISA Development -
Corneal Mesenchymal Stromal Cells Are Directly Antiangiogenic via PEDF and sFLT-1
Authors: M Eslani, I Putra, X Shen, J Hamouie, N Afsharkham, S Besharat, MI Rosenblatt, R Dana, P Hematti, AR Djalilian
Invest. Ophthalmol. Vis. Sci., 2017-10-01;58(12):5507-5517.
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