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Mouse/Rat WNK1 Antibody

Catalog #: AF2849
5 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF2849
AF2849-SP
Detection of Mouse/Rat WNK1 by Western Blot.
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Citations (5)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse/Rat WNK1 Antibody Summary

Species Reactivity
Mouse, Rat
Specificity
Detects mouse and rat WNK1 in Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant rat WNK1
Ser816-Ser1173
Accession # Q9JIH7
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse/Rat WNK1 antibody by Western Blot. View Larger

Detection of Mouse/Rat WNK1 by Western Blot. Western blot shows lysates of DA3 mouse myeloma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Mouse/Rat WNK1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2849) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for WNK1 at approximately 230 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: WNK1

With No Lysine (K) 1 (WNK1) belongs to a novel family of serine/threonine protein kinases involved in regulating ion transport. The WNKs contain a cysteine instead of the usual lysine in their kinase activation domains. WNK1 mutations have been implicated in Pseudohypoaldosteronism type II (PHAII), an autosomal dominant disorder characterized by hypertension, increased renal salt absorption, and impaired potassium and hydrogen excretion.

Long Name
WNK Lysine-deficient Protein Kinase 1
Entrez Gene IDs
65125 (Human); 232341 (Mouse); 116477 (Rat)
Alternate Names
EC 2.7.11; EC 2.7.11.1; Erythrocyte 65 kDa protein; HSAN2hereditary sensory neuropathy, type II; HSN2MGC163339; hWNK1; KDP; KDPMGC163341; KIAA0344; Kinase deficient protein; p65; PHA2C; PRKWNK1; PRKWNK1PSK; prostate-derived sterile 20-like kinase; Protein kinase lysine-deficient 1; Protein kinase with no lysine 1; protein kinase, lysine deficient 1; serine/threonine-protein kinase WNK1; WNK lysine deficient protein kinase 1; WNK1

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Citations for Mouse/Rat WNK1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. WNK1 enforces macrophage lineage fidelity
    Authors: Trzeciak, AJ;Rojas, WS;Liu, ZL;Krebs, AS;Wang, Z;Saavedra, PHV;Miranda, IC;Lipshutz, A;Xie, J;Huang, CL;Overholtzer, M;Glickman, MS;Parkhurst, CN;Vierbuchen, T;Lucas, CD;Perry, JSA;
    bioRxiv : the preprint server for biology
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. WNK1 promotes water homeostasis by acting as a central osmolality sensor for arginine vasopressin release
    Authors: Jin X, Xie J, Yeh CW et al.
    The Journal of clinical investigation
  3. WNK1 is a chloride-stimulated scaffold that regulates mTORC2-activity and ion transport
    Authors: B Saha, DCAL Dellova, J Demko, MV Sørensen, E Takagi, CE Gleason, W Shabbir, D Pearce
    Journal of Cell Science, 2022-12-05;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Phosphatidic acid-dependent localization and basal de-phosphorylation of RA-GEFs regulate lymphocyte trafficking
    Authors: S Ishihara, T Sato, G Du, D Guardavacc, A Nakajima, S Sawai, T Kataoka, K Katagiri
    BMC Biol., 2020-06-29;18(1):75.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Mutations in the nervous system--specific HSN2 exon of WNK1 cause hereditary sensory neuropathy type II.
    Authors: Shekarabi M, Girard N, Riviere JB, Dion P, Houle M, Toulouse A, Lafreniere RG, Vercauteren F, Hince P, Laganiere J, Rochefort D, Faivre L, Samuels M, Rouleau GA
    J. Clin. Invest., 2008-07-01;118(7):2496-505.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot

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