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Mouse Reg1 Antibody

Catalog #: AF1657
3 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF1657
AF1657-SP
Product Details
Citations (3)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Reg1 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Reg1 in direct ELISAs and Western blots. In Western blots, approximately 10% cross-reactivity with recombinant mouse (rm) Reg2 is observed and less than 5% cross-reactivity with rmReg3A, recombinant human (rh) Reg1B, rhReg4, recombinant rat (rr) Reg3 and rrReg2 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse Reg1
Gln22-Gly165
Accession # P43137
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Reg1

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Reg1

Reg1, also known as pancreatic thread protein (PTP), pancreatic stone protein (PSP) and lithostathine, is the founding member of the Reg family of proteins, which are secreted proteins with a C-type lectin domain. Reg1 is expressed during islet regeneration. Reg1 functions in an autocrine/paracrine fashion via a cell surface Reg receptor that mediates a growth signal for beta -cell regeneration (1).

References
  1. Okamoto, H. and S. Takasawa (2002) Diabetes 51:5462.
Long Name
Regenerating Islet-derived 1
Entrez Gene IDs
19692 (Mouse)
Alternate Names
ICRF; Islet cells regeneration factor; Islet of Langerhans regenerating protein; lithostathine-1-alpha; Pancreatic stone protein; pancreatic stone protein, secretory; Pancreatic thread protein; protein-X; PSPregenerating islet-derived 1 alpha (pancreatic stone protein, pancreatic threadprotein); PSPS1REG-1-alpha; PSPSMGC12447; PTPP19; Reg1; regenerating islet-derived 1 alpha; Regenerating islet-derived protein 1-alpha; Regenerating protein I alpha; REGlithostathine 1 alpha

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Citations for Mouse Reg1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Genetic and pharmacologic inhibition of ALDH1A3 as a treatment of beta-cell failure
    Authors: J Son, W Du, M Esposito, K Shariati, H Ding, Y Kang, D Accili
    Nature Communications, 2023-02-02;14(1):558.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. Proteomic analysis of AQP11-null kidney: Proximal tubular type polycystic kidney disease
    Authors: T Saito, Y Tanaka, Y Morishita, K Ishibashi
    Biochem Biophys Rep, 2017-11-23;13(0):17-21.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  3. Reg-II is an exocrine pancreas injury-response product that is up-regulated by keratin absence or mutation.
    Authors: Zhong B, Strnad P, Toivola DM, Tao GZ, Ji X, Greenberg HB, Omary MB
    Mol. Biol. Cell, 2007-09-26;18(12):4969-78.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot

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