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Mouse Reg3B Antibody

Catalog #: MAB5110
9 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB5110
MAB5110-SP
Detection of Mouse Reg3B by Western Blot.
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Product Details
Citations (9)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Reg3B Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Reg3B in direct ELISAs and Western blots. In direct ELISAs, does not cross-react with recombinant mouse (rm) Reg3A, rmReg3D, recombinant human Reg3D, recombinant rat (rr) Reg3B, or rrReg3G.
Source
Monoclonal Rat IgG2B Clone # 518630
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant mouse Reg3B

Glu27-Gly175
Accession # P35230
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse Reg3B antibody by Western Blot. View Larger

Detection of Mouse Reg3B by Western Blot. Western blot shows lysates of mouse small intestine tissue. PVDF membrane was probed with 2 µg/mL of Mouse Reg3B Monoclonal Antibody (Catalog # MAB5110) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Reg3B at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Reg3B

Reg3B (regenerating islet-derived protein III beta; also PAP-I and HIP) is a secreted, 17 kDa member of the Reg gene family, C-type lectin gene superfamily.  It is found in pancreatic acinar cells, intestinal enterocytes and developing spinal motoneurons.  Reg3B may play a role in intestinal epithelial cell renewal. It contains one C-type lectin domain.  Mature mouse Reg3B is 91% and 67% aa identical to rat and human Reg3B, respectively.     

Long Name
Regenerating Islet-derived IIIb
Entrez Gene IDs
18489 (Mouse); 24618 (Rat)
Alternate Names
PAP1; Reg3B

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Citations for Mouse Reg3B Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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  1. Intestinally Secreted C-Type Lectin Reg3b Attenuates Salmonellosis but Not Listeriosis in Mice
    Authors: Marleen T. J. van Ampting, Linda M. P. Loonen, Arjan J. Schonewille, Irene Konings, Carolien Vink, Juan Iovanna et al.
    Infection and Immunity
  2. Helicobacter pylori-induced Rev-erb&alpha fosters gastric bacteria colonization by impairing host innate and adaptive defense
    Authors: FY Mao, YP Lv, CJ Hao, YS Teng, YG Liu, P Cheng, SM Yang, W Chen, T Liu, QM Zou, R Xie, JY Xu, Y Zhuang
    Cellular and Molecular Gastroenterology and Hepatology, 2021-03-03;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  3. Supplementation of endogenous Ahr ligands reverses insulin resistance and associated inflammation in an insulin-dependent diabetic mouse model
    Authors: WC Liu, PH Chen, LW Chen
    J. Nutr. Biochem., 2020-04-01;83(0):108384.
    Species: Mouse
    Sample Types: Protein
    Applications: Western Blot
  4. Lactobacillus salivarius reverse antibiotic-induced lung defense impairment in a ventilator model
    Authors: TB Tsay, MC Yang, WH Chang, PH Chen, LW Chen
    J Transl Med, 2018-08-13;16(1):225.
    Species: Mouse
    Sample Types:
  5. The Role of Intestinal C-type Regenerating Islet Derived-3 Lectins for Nonalcoholic Steatohepatitis
    Authors: S Bluemel, L Wang, C Martino, S Lee, Y Wang, B Williams, A Horvath, V Stadlbauer, K Zengler, B Schnabl
    Hepatol Commun, 2018-02-28;2(4):393-406.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  6. YAP/TAZ-Dependent Reprogramming of Colonic Epithelium Links ECM Remodeling to Tissue Regeneration
    Authors: S Yui, L Azzolin, M Maimets, MT Pedersen, RP Fordham, SL Hansen, HL Larsen, J Guiu, MRP Alves, CF Rundsten, JV Johansen, Y Li, CD Madsen, T Nakamura, M Watanabe, OH Nielsen, PJ Schweiger, S Piccolo, KB Jensen
    Cell Stem Cell, 2017-12-14;22(1):35-49.e7.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  7. Toll-like receptor stimulation induces nondefensin protein expression and reverses antibiotic-induced gut defense impairment.
    Authors: Wu Y, Hsu C, Chen P, Fung C, Chen L
    Infect Immun, 2014-03-04;82(5):1994-2005.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  8. Diabetes aggravates acute pancreatitis and inhibits pancreas regeneration in mice.
    Authors: Zechner D, Spitzner M, Bobrowski A, Knapp N, Kuhla A, Vollmar B
    Diabetologia, 2012-02-12;55(5):1526-34.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Tissue
    Applications: IHC, Western Blot
  9. Interleukin-22 promotes intestinal-stem-cell-mediated epithelial regeneration
    Authors: Caroline A. Lindemans, Marco Calafiore, Anna M. Mertelsmann, Margaret H. O’Connor, Jarrod A. Dudakov, Robert R. Jenq et al.
    Nature

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