Mouse Renin 1 Propeptide Antibody

Catalog #: MAB4277 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB4277
MAB4277-SP

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Mouse Renin 1 Propeptide Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Renin 1 Propeptide in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with the mature form of mouse Renin 1, recombinant human Renin, recombinant mouse (rm) Cathepsin D, or rmCathepsin E is observed.

Source

Monoclonal Rat IgG_2A Clone # 434717

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Renin 1
Leu22-Arg402
Accession # P06281

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Mouse Renin (Catalog # 4277-AS)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Renin

Mouse Renin is a secreted, 42‑47 kDa glycosylated member of the peptidase A1 family. It is an aspartyl protease that cleaves angiotensinogen to form angiotensin I. In mouse, there are two genes that code for Renin. One is in the submandibular gland and the other is in the kidney. The two mature Renin molecules are 95% amino acid (aa) identical. Renal Renin (Renin‑1) is synthesized as a 381 aa proform (aa 22‑402). In the kidney, pro‑Renin is proteolytically cleaved after Thr71 to generate a mature enzyme. Mouse pro‑Renin shares 70% and 85% aa sequence identity with human and rat pro‑Renin, respectively.

Entrez Gene IDs

5972 (Human); 19701 (Mouse)

Alternate Names

angiotensin-forming enzyme; Angiotensinogenase; EC 3.4.23; EC 3.4.23.15; FLJ10761; HNFJ2; Prorenin; REN; renin precursor, renal; Renin