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Mouse Resistin Quantikine ELISA Kit

Catalog #: MRSN00
20 Citations Datasheet / COA / SDS
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MRSN00
Mouse Resistin ELISA Standard Curve
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Citations (20)
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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Mouse Resistin Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)
Sensitivity
8 pg/mL
Assay Range
31.2 - 2,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant mouse Resistin
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Mouse Resistin Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse Resistin in cell culture supernates, mouse serum, and plasma. It contains E. coli-expressed recombinant mouse Resistin and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural mouse Resistin showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring mouse Resistin.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 41 41 41
Mean (pg/mL) 84 245 494 84 239 508
Standard Deviation 3.4 7.2 13.3 6.1 16.4 34.9
CV% 4 2.9 2.7 7.3 6.9 6.9

Recovery

The recovery of mouse Resistin spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=8) 99 85-108
EDTA Plasma (n=8) 97 87-107
Heparin Plasma (n=8) 102 89-113
Serum (n=6) 99 93-108

Linearity

To assess the linearity of the assay, samples containing high concentrations of mouse Resistin in each matrix were diluted with Calibrator Diluent and then assayed.
Mouse Resistin ELISA Linearity

Scientific Data

N/A Mouse Resistin ELISA Standard Curve View Larger

Mouse Resistin ELISA Standard Curve

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Resistin

Resistin, also known ADSF and FIZZ3, is a secreted peptide hormone that circulates as either a noncovalent trimer, or a disulfide-linked homohexamer. Resistin is expressed by white adipocytes, splenocytes, astrocytes, and anterior pituitary epithelium. It blocks Insulin-stimulated uptake of glucose by adipocytes and promotes glucose release by hepatocytes. High circulating Insulin levels induce Resistin secretion which then antagonize Insulin action.

Entrez Gene IDs:
56729 (Human); 57264 (Mouse)
Alternate Names:
Adipose tissue-specific secretory factor; ADSF; ADSFMGC126609; C/EBP-epsilon regulated myeloid-specific secreted cysteine-rich proteinprecursor 1; C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein; Cysteine-rich secreted protein A12-alpha-like 2; Cysteine-rich secreted protein FIZZ3; FIZZ3; FIZZ3MGC126603; found in inflammatory zone 3; HXCP1; Resistin; RETN; RETN1; RSTNXCP1
&#9888; WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 50 µL Assay Diluent
  3.   Add 50 µL of Assay Diluent to each well.
    4. 50 µL Standard, Control, or Sample
  4.   Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
  5.   Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.
    6. 100 µL Conjugate
  6.   Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
  7.   Aspirate and wash 5 times.
    8. 100 µL Substrate Solution
  8.   Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.
    9. 100 µL Stop Solution
  9.   Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Mouse Resistin Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

20 Citations: Showing 1 - 10
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  1. Blockade of CXXC5-dishevelled interaction inhibits adipogenic differentiation, obesity, and insulin resistance in mice
    Authors: SH Seo, D Lee, SH Lee, KY Choi
    Scientific Reports, 2022-11-30;12(1):20669.
    Species: Mouse
    Sample Types: Serum
  2. Discovery of a potent GIPR peptide antagonist that is effective in rodent and human systems
    Authors: B Yang, VM Gelfanov, K El, A Chen, R Rohlfs, B DuBois, AM Kruse Hans, D Perez-Tilv, PJ Knerr, D D'Alessio, JE Campbell, JD Douros, B Finan
    Molecular Metabolism, 2022-11-15;0(0):101638.
    Species: Mouse
    Sample Types: Serum
  3. REDD1 promotes obesity-induced metabolic dysfunction via atypical NF-kappaB activation
    Authors: DK Lee, T Kim, J Byeon, M Park, S Kim, J Kim, S Choi, G Lee, C Park, KW Lee, YJ Kwon, JH Lee, YG Kwon, YM Kim
    Nature Communications, 2022-10-22;13(1):6303.
    Species: Mouse
    Sample Types: Plasma
  4. Polygonatum sibiricum F. Delaroche polysaccharide ameliorates HFD?induced mouse obesity via regulation of lipid metabolism and inflammatory response
    Authors: B Liu, Y Tang, Z Song, J Ge
    Molecular Medicine Reports, 2021-05-13;24(1):.
    Species: Mouse
    Sample Types: Plasma
  5. Prostaglandin E2-EP4 Axis Promotes Lipolysis and Fibrosis in Adipose Tissue Leading to Ectopic Fat Deposition and Insulin Resistance
    Authors: T Inazumi, K Yamada, N Shirata, H Sato, Y Taketomi, K Morita, H Hohjoh, S Tsuchiya, K Oniki, T Watanabe, Y Sasaki, Y Oike, Y Ogata, J Saruwatari, M Murakami, Y Sugimoto
    Cell Rep, 2020-10-13;33(2):108265.
    Species: Mouse
    Sample Types: Serum
  6. Depletion of B cell-activating factor attenuates hepatic fat accumulation in a murine model of nonalcoholic fatty liver disease
    Authors: Y Nakamura, M Abe, K Kawasaki, T Miyake, T Watanabe, O Yoshida, M Hirooka, B Matsuura, Y Hiasa
    Sci Rep, 2019-01-30;9(1):977.
    Species: Mouse
    Sample Types: Serum
  7. Leptin Production by Encapsulated Adipocytes Increases Brown Fat, Decreases Resistin, and Improves Glucose Intolerance in Obese Mice
    Authors: DJ DiSilvestr, E Melgar-Ber, R Yasmeen, P Fadda, LJ Lee, A Kalyanasun, CL Gilor, O Ziouzenkov
    PLoS ONE, 2016-04-07;11(4):e0153198.
    Species: Mouse
    Sample Types: Plasma
  8. sigma1B adaptin regulates adipogenesis by mediating the sorting of sortilin in adipose tissue.
    Authors: Baltes J, Larsen J, Radhakrishnan K, Geumann C, Kratzke M, Petersen C, Schu P
    J Cell Sci, 2014-06-13;127(0):3477-87.
    Species: Mouse
    Sample Types: Serum
  9. PKClambda haploinsufficiency prevents diabetes by a mechanism involving alterations in hepatic enzymes.
    Authors: Sajan M, Ivey R, Lee M, Mastorides S, Jurczak M, Samuels V, Shulman G, Braun U, Leitges M, Farese R
    Mol Endocrinol, 2014-05-30;28(7):1097-107.
    Species: Mouse
    Sample Types: Serum
  10. Induction of adipocyte hyperplasia in subcutaneous fat depot alleviated type 2 diabetes symptoms in obese mice.
    Authors: Lu Q, Li M, Zou Y, Cao T
    Obesity (Silver Spring), 2014-02-11;22(7):1623-31.
    Species: Mouse
    Sample Types: Serum
  11. Ox-LDL induces ER stress and promotes the adipokines secretion in 3T3-L1 adipocytes.
    Authors: Chen Y, Chen M, Wu Z, Zhao S
    PLoS ONE, 2013-11-22;8(10):e81379.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  12. Tangeretin stimulates glucose uptake via regulation of AMPK signaling pathways in C2C12 myotubes and improves glucose tolerance in high-fat diet-induced obese mice.
    Mol. Cell. Endocrinol., 2012-03-28;358(1):127-34.
    Species: Mouse
    Sample Types: Serum
  13. HCV core-induced nonobese hepatic steatosis is associated with hypoadiponectinemia and is ameliorated by adiponectin administration.
    Authors: Chang ML, Yeh HC, Tsou YK
    Obesity (Silver Spring), 2012-02-23;20(7):1474-80.
    Species: Mouse
    Sample Types: Serum
  14. Castration-induced changes in mouse epididymal white adipose tissue.
    Authors: Floryk D, Kurosaka S, Tanimoto R, Yang G, Goltsov A, Park S, Thompson TC
    Mol. Cell. Endocrinol., 2011-07-12;345(1):58-67.
    Species: Mouse
    Sample Types: Serum
  15. eNOS plays a major role in adiponectin synthesis in adipocytes.
    Authors: Koh EH, Kim M, Ranjan KC, Kim HS, Park HS, Oh KS, Park IS, Lee WJ, Kim MS, Park JY, Youn JH, Lee KU
    Am. J. Physiol. Endocrinol. Metab., 2010-02-02;298(4):E846-53.
    Species: Mouse
    Sample Types: Plasma
  16. PPARgamma in the endothelium regulates metabolic responses to high-fat diet in mice.
    Authors: Kanda T, Brown JD, Orasanu G, Vogel S, Gonzalez FJ, Sartoretto J, Michel T, Plutzky J
    J. Clin. Invest., 2008-12-08;119(1):110-24.
    Species: Mouse
    Sample Types: Serum
  17. Regulation of the brown and white fat gene programs through a PRDM16/CtBP transcriptional complex.
    Authors: Kajimura S, Seale P, Tomaru T, Erdjument-Bromage H, Cooper MP, Ruas JL, Chin S, Tempst P, Lazar MA, Spiegelman BM
    Genes Dev., 2008-05-15;22(10):1397-409.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  18. Role and regulation of adipokines during zymosan-induced peritoneal inflammation in mice.
    Authors: Pini M, Gove ME, Sennello JA, van Baal JW, Chan L, Fantuzzi G
    Endocrinology, 2008-05-01;149(8):4080-5.
    Species: Mouse
    Sample Types: Serum
  19. Octylphenol stimulates resistin gene expression in 3T3-L1 adipocytes via the estrogen receptor and extracellular signal-regulated kinase pathways.
    Authors: Lee MJ, Lin H, Liu CW, Wu MH, Liao WJ, Chang HH, Ku HC, Chien YS, Ding WH, Kao YH
    Am. J. Physiol., Cell Physiol., 2008-04-16;294(6):C1542-51.
    Species: Mouse
    Sample Types: Serum
  20. Neuropeptide Y acts directly in the periphery on fat tissue and mediates stress-induced obesity and metabolic syndrome.
    Authors: Kuo LE, Kitlinska JB, Tilan JU, Li L, Baker SB, Johnson MD, Lee EW, Burnett MS, Fricke ST, Kvetnansky R, Herzog H, Zukowska Z
    Nat. Med., 2007-07-01;13(7):803-11.
    Species: Mouse
    Sample Types: Plasma

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