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Mouse RGM-B Biotinylated Antibody

Catalog #: BAF3597
2 Citations Datasheet / COA / SDS
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BAF3597
RGM‑B in Neuro‑2A Mouse Cell Line.
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Citations (2)
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Mouse RGM-B Biotinylated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse RGM‑B in Western blots. In Western blots, approximately 5% cross-reactivity with recombinant mouse (rm) RGM-C is observed and less than 2% cross‑reactivity with rmRGM‑A is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse RGM-B
Gly49-Ser414
Accession # Q7TQ33
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse RGM-B (Catalog # 3597-RG)
Flow Cytometry
0.25 µg/106 cells
Neuro‑2A mouse neuroblastoma cell line
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry RGM-B antibody in Neuro-2A Mouse Cell Line by Immunocytochemistry (ICC). View Larger

RGM‑B in Neuro‑2A Mouse Cell Line. RGM-B was detected in immersion fixed Neuro-2A mouse neuroblastoma cell line using Sheep Anti-Mouse RGM-B Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF3597) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: RGM-B

RGM-B, also known as DRAGON, is a 40 kDa member of the repulsive guidance molecule (RGM) family of GPI-linked neuronal and muscle membrane proteins (1, 2). It is synthesized as a preproprotein that consists of a 48 amino acid (aa) signal sequence, a 367 aa mature region, and a 21 aa C-terminal prosegment (3). RGM-B contains two potential N-linked glycosylation sites and an abbreviated von Willebrand factor domain. Potential proteolytic cleavage within the VWF domain is supported by R&D Systems’ in house data (4). Within the region following the VWF domain, mouse RGM-B shares 49% and 43% aa sequence identity with RGM-A and RGM-C, respectively. It shares 90%, 79%, 92%, and 93% aa sequence identity with bovine, chicken, human, and rhesus macaque RGM-B, respectively. RGM-B is expressed in the developing and adult nervous system, particularly in the dorsal root ganglia and mantle layer of the spinal cord (3-5). In mouse, it shows a complementary, non-overlapping distribution with RGM-A (2-5). RGM-B is also expressed in fetal and adult enteric ganglia and in postnatal intestinal epithelium (6). RGM-B expression has been detected in neuronal cell bodies and proximal axonal segments (4) but is also present on the cell surface, where it interacts homophilically and mediates neuronal adhesion (3). RGM-B additionally functions as a BMP coreceptor. It directly binds BMP-2 and -4 but not other TGF-beta family proteins (7). RGM-B associates with BMP type I (ALK-2, -3, -6) and type II (Activin RIIA, Activin RIIB) receptors and enhances BMP signaling (7).

References
  1. Monnier, P.P. et al. (2002) Nature 419:392.
  2. Schmidtmer, J. and D. Engelkamp (2004) Gene Exp. Patterns 4:105.
  3. Samad, T.A. et al. (2004) J. Neurosci. 24:2027.
  4. Niederkofler, V. et al. (2004) J. Neurosci. 24:808.
  5. Oldekamp, J. et al. (2004) Gene Exp. Patterns 4:283.
  6. Metzger, M. et al. (2005) Dev. Dyn. 234:169.
  7. Samad, T.A. et al. (2005) J. Biol. Chem. 280:14122.
Long Name
Repulsive Guidance Molecule B
Entrez Gene IDs
285704 (Human); 68799 (Mouse)
Alternate Names
DKFZp434P228; Dragon; DRG11-responsive axonal guidance and outgrowth of neurite; RGM domain family, member B; RGMB; RGM-B

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Citations for Mouse RGM-B Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Targeting PD-L2-RGMb overcomes microbiome-related immunotherapy resistance
    Authors: Park, JS;Gazzaniga, FS;Wu, M;Luthens, AK;Gillis, J;Zheng, W;LaFleur, MW;Johnson, SB;Morad, G;Park, EM;Zhou, Y;Watowich, SS;Wargo, JA;Freeman, GJ;Kasper, DL;Sharpe, AH;
    Nature
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. B7-DC (PD-L2) costimulation of CD4(+) T-helper 1 response via RGMb
    Authors: X Nie, W Chen, Y Zhu, B Huang, W Yu, Z Wu, S Guo, Y Zhu, L Luo, S Wang, L Chen
    Cell. Mol. Immunol., 2017-05-08;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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