Mouse S1P5/EDG-8 PE-conjugated Antibody

Catalog #: FAB9084P Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
FAB9084P-025

Detection of S1P5/EDG‑8 in HEK293 Human Cell Line Transfected with Mouse S1P5/EDG-8 and eGFP by Flow Cytometry.

1 Image

All S1P5/EDG-8 Antibodies

Product Details

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse S1P5/EDG-8 PE-conjugated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse S1P₅/EDG-8 in Western blots.

Source

Recombinant Monoclonal Rabbit IgG Clone # 1196A

Purification

Protein A or G purified from cell culture supernatant

Immunogen

Mouse S1P₅/EDG-8 peptide corresponding to the N-terminal extracellular sequence
Accession # Q91X56

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

View Larger

Detection of S1P₅/EDG‑8 in HEK293 Human Cell Line Transfected with Mouse S1P₅/EDG-8 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with mouse S1P₅/EDG-8 and eGFP were stained with either (A) Rabbit Anti-Mouse S1P₅/EDG‑8 PE‑conjugated Monoclonal Antibody (Catalog # FAB9084P) or (B) Normal Rabbit IgG Phycoerythrin Control (Catalog # IC1051P). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: S1P5/EDG-8

S1P₅ (Sphingosine-1 Phosphate receptor 5), also known as EDG-8 (Endothelial Differentiation Gene 8), is a 43-45 kDa member of the EDG family, S1P-binding subfamily of GPCRs. Along with S1P₁-S1P₄ (or EDG-1, EDG-5, EDG-3 and EDG-6, respectively), S1P₅ is known to bind S1P, a lipid synthesized by platelets, neutrophils, smooth muscle cells, mast cells and select fibroblasts. Mouse S1P₅ is a 400 amino acid (aa) 7-transmembrane glycoprotein that is expressed on brain endothelium, renal mesangial cells, Ly6C⁺ (in human CD14⁺CD16^-) monocytes, pre- and mature oligodendrocytes, and CD27^-CD116⁺ (mature) NK cells. S1P₅ appears to play a role in cell trafficking. On monocytes and NK cells, S1P₅ promotes cell migration out of the bone marrow, a phenomenon that may not be related to an S1P concentration gradient. S1P₅ also helps maintain the integrity of the blood-brain-barrier. On oligodendrocytes and precursors, S1P₅ appears to regulate cell survival and pseudopod extension. The extracellular segment of mouse S1P₅ shares 96% and 94% aa sequence identity with rat and human S1P₅ extracellular domains, respectively.

Long Name

Endothelial Differentiation Gene 8

Entrez Gene IDs

53637 (Human); 94226 (Mouse); 60399 (Rat)

Alternate Names

EDG8; Edg-8; EDG8S1P receptor Edg-8; Endothelial differentiation G-protein-coupled receptor 8; endothelial differentiation, sphingolipid G-protein-coupled receptor, 8; LPB4; S1P receptor 5; S1P5; S1PR5; sphingosine 1-phosphate receptor 5; sphingosine 1-phosphate receptor EDG8; Sphingosine 1-phosphate receptor Edg-8; sphingosine-1-phosphate receptor 5; SPPR-1; SPPR-2