Mouse SDNSF/MCFD2 Antibody

Catalog #: MAB2556 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB2556
MAB2556-SP

All SDNSF/MCFD2 Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse SDNSF/MCFD2 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse SDNSF/MCFD2 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human SDNSF is observed.

Source

Monoclonal Rat IgG_2A Clone # 300812

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse SDNSF/MCFD2
His27-Gln145 (predicted)
Accession # Q8K5B2

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Immunohistochemistry

8-25 µg/mL

Immersion fixed frozen sections of embryonic mouse brain

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SDNSF/MCFD2

SDNSF, also known as MCFD2 (multiple coagulation factor deficiency 2), is a secreted molecule from adult hippocampal neural stem/progenitor cells (ANSC) that functions as an autocrine/paracrine factor to maintain neurogenesis in the central nervous system. It is also a component of the MCDF2-LMAN1 complex that functions as a specific cargo receptor for the ER to Golgi transport of proteins. Mutations in MCFD2 causes factor 5 and factor 8 combined deficiency.

Long Name

Stem Cell-derived Neuronal Stem/Progenitor Cell Supporting Factor

Entrez Gene IDs

90411 (Human); 193813 (Mouse)

Alternate Names

DKFZp686G21263; F5F8D2; LMAN1IP; MCFD2; multiple coagulation factor deficiency 2; multiple coagulation factor deficiency protein 2; neural stem cell derived neuronal survival protein; Neural stem cell-derived neuronal survival protein; SDNSF; SDNSFF5F8D