Mouse SDNSF/MCFD2 Antibody

Catalog #: AF2556 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF2556
AF2556-SP

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Mouse SDNSF/MCFD2 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse SDNSF/MCFD2 in direct ELISAs and Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse SDNSF/MCFD2
His27-Gln145
Accession # Q8K5B2

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse SDNSF/MCFD2

Immunohistochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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SDNSF/MCFD2 in Embryonic Mouse Brain. SDNSF/MCFD2 was detected in immersion fixed frozen sections of embryonic mouse brain (E10, neural tube) using Goat Anti-Mouse SDNSF/MCFD2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2556) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to secretion. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SDNSF/MCFD2

SDNSF, also known as MCFD2 (multiple coagulation factor deficiency 2), was described as a secreted molecule from adult hippocampal neural stem/progenitor cells (ANSC) that functions as an autocrine/paracrine factor to maintain neurogenesis in the central nervous system. It is also a component of the MCDF2-LMAN1 (Mannose-binding lectin-1, also known as ERGIC-53) complex that functions as a specific cargo receptor for the ER to golgi transport of proteins. Mutations in MCFD2 causes factor 5 and factor 8 combined deficiency. Human SDNSF shares 84% and 82% amino acid sequence identity with rat and mouse SDNSF, respectively.

Long Name

Stem Cell-derived Neuronal Stem/Progenitor Cell Supporting Factor

Entrez Gene IDs

90411 (Human); 193813 (Mouse)

Alternate Names

DKFZp686G21263; F5F8D2; LMAN1IP; MCFD2; multiple coagulation factor deficiency 2; multiple coagulation factor deficiency protein 2; neural stem cell derived neuronal survival protein; Neural stem cell-derived neuronal survival protein; SDNSF; SDNSFF5F8D