Mouse Semaphorin 3B Antibody Summary
Pro26-Pro722
Accession # Q62177
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Mouse Semaphorin 3B by Western Blot. Western blot shows lysates of NS0 mouse myeloma cell line transfected with mouse Semaphorin 3B. PVDF membrane was probed with 0.5 µg/mL of Rat Anti-Mouse Semaphorin 3B Monoclonal Antibody (Catalog # MAB5440) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Semaphorin 3B at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Semaphorin 3B in NS0 Mouse Cell Line. Semaphorin 3B was detected in immersion fixed NS0 mouse myeloma cell line transfected with mouse Semaphorin 3B (upper panel) or irrelevant transfectant (lower panel) using Rat Anti-Mouse Semaphorin 3B Monoclonal Antibody (Catalog # MAB5440) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Semaphorin 3B
Sema3b (Semaphorin 3B) is a 748 aa secreted glycoprotein. Mouse Sema3b shares 88% and 96% aa identity with human and rat Sema3b, respectively. The semaphorin family has been demonstrated to play a role in axon guidance. Additionally, Sema3b has been implicated in bone mass regulation and tumor suppression.
Product Datasheets
Citation for Mouse Semaphorin 3B Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Regulation of antitumour CD8 T-cell immunity and checkpoint blockade immunotherapy by Neuropilin-1
Authors: M Leclerc, E Voilin, G Gros, S Corgnac, V de Montpré, P Validire, G Bismuth, F Mami-Choua
Nat Commun, 2019-07-26;10(1):3345.
Species: Mouse
Sample Types: Protein
Applications: Western Blot
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