Mouse Serpin A1a Antibody

Catalog #: MAB7690
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB7690
MAB7690-SP

Detection of Mouse SerpinA1a by Western Blot.

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All Serpin A1a Antibodies

Product Details

Citations (2)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Serpin A1a Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Serpin A1a in ELISAs. In direct ELISAs, 100% cross-reactivity with recombinant mouse (rm) Serpin A1c, approximately 15% cross-reactivity with recombinant human A1, and less than 5% cross-reactivity with rmSerpin A3n is observed.

Source

Monoclonal Rat IgG₁ Clone # 882213

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Serpin A1a
Met1-Lys413
Accession # P07758

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.2 µg/mL

See below

Simple Western

2 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Mouse SerpinA1a by Western Blot. Western blot shows mouse serum. PVDF membrane was probed with 0.2 µg/mL of Rat Anti-Mouse Serpin A1a Monoclonal Antibody (Catalog # MAB7690) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Serpin A1a at approximately 50-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western

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Detection of Mouse Serpin A1a by Simple Western^TM. Simple Western lane view shows mouse serum, loaded at 0.2 mg/mL. A specific band was detected for Serpin A1a at approximately 62 kDa (as indicated) using 2 µg/mL of Rat Anti-Mouse Serpin A1a Monoclonal Antibody (Catalog # MAB7690) followed by 1:50 dilution of HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Serpin A1a

Serpin A1a, also known as alpha-1-antitrypsin, belongs to the serpin superfamily of proteinase inhibitors. It is one of five alpha 1-proteinase inhibitors produced in mice. Serpin A1a is produced in the liver, then secreted into the bloodstream. It inhibits a number of serine proteases, including elastase, trypsin, plasmin, and thrombin (1, 2). Serpin A1a’s major function is to protect the lungs from neutrophil elastase. Serpin A1a deficiency can cause complications such as emphysema, and chronic obstructive pulmonary disease (COPD).

References

Gettins, P.G. (2002) Chem. Rev. 102:4751.
Kalsheker N.A. (1989) Biosci. Rep. 9:129. 3. Ritchie, R.F. et al. (2000) J. Clin. Lab. Anal. 14:284.

Entrez Gene IDs

5265 (Human); 20700 (Mouse)

Alternate Names

A1A; A1AT; AAT; Aat2; alpha1AT; Dom1; nNIF; PI; PI1; PRO2275; Serpin A1a; Spi1-1

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Mouse Serpin A1a Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Serpin neuropathology in the P497S UBQLN2 mouse model of ALS/FTD
Authors: Higgins NR, Greenslade JE, Wu JJ et al.
Brain pathology (Zurich, Switzerland)
Rosa26-LSL-dCas9-VPR: a versatile mouse model for tissue specific and simultaneous activation of multiple genes for drug discovery
Authors: D Pakalnišky, T Schönberge, B Strobel, B Stierstorf, T Lamla, M Schuler, M Lenter
Scientific Reports, 2022-11-10;12(1):19268.
Species: Transgenic Mouse
Sample Types: Tissue Homogenates
Applications: Simple Western