Mouse Siglec-1/CD169 PE-conjugated Antibody Summary
Thr20-Leu1639 (predicted)
Accession # Q62230
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Siglec‑1/CD169 in RAW 264.7 Mouse Cell Line by Flow Cytometry. RAW 264.7 mouse monocyte/macrophage cells stimulated with 1ug/ml LPS overnight were stained with Rat Anti-Mouse Siglec-1/CD169 PE-conjugated Monoclonal Antibody (Catalog # FAB5610P, filled histogram) or isotype control antibody (Catalog # IC006P, open histogram). View our protocol for Staining Membrane-associated Proteins.
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Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Siglec-1/CD169
Siglec-1, also known as Sialoadhesin or CD169, is a 175-185 kDa type I transmembrane glycoprotein belonging to the Siglec family of sialic acid specific I-type lectins within the immunoglobulin superfamily. Mouse Siglec-1 contains a 1619 amino acid (aa) extracellular domain (ECD) with one Ig-like V-set domain and 16 Ig-like C2-set domains. The ECD shares 73% and 83% aa sequence identity with human and rat Siglec-1, respectively. Alternate splicing generates two soluble isoforms containing either 16 or the first 3 Ig-like domains. Siglec-1 is expressed by some tissue macrophages, dendritic cells and circulating monocytes during certain infections. It binds sialylated molecules including MMR, MGL1/CD301a, MUC1, PSGL-1 and CD43.
Product Datasheets
Citations for Mouse Siglec-1/CD169 PE-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 2
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LECT2 drives haematopoietic stem cell expansion and mobilization via regulating the macrophages and osteolineage cells
Nat Commun, 2016-09-06;7(0):12719.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Induction of Siglec-1 by Endotoxin Tolerance Suppresses the Innate Immune Response by Promoting TGF-?1 Production
Authors: Y Wu, C Lan, D Ren, GY Chen
J. Biol. Chem., 2016-04-18;291(23):12370-82.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
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Culture cells were washed in Flow Cytometry staining
buffer, and incubated for 1 h on ice with directly conjugated-antibodies in flow cytometry staining buffer. We also knockdown Siglec-1 with shrna.
