Home / Stella/Dppa3 / Mouse Stella/Dppa3 Antibody

Mouse Stella/Dppa3 Antibody

Catalog #: MAB2566
3 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB2566
MAB2566-SP
Product Details
Citations (3)
FAQs
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Stella/Dppa3 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Stella/Dppa3 in direct ELISAs and Western blots.
Source
Monoclonal Rat IgG2A Clone # 283910
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant mouse Stella/Dppa3
Met1-Asn150
Accession # Q8QZY3
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse Stella/Dppa3
Immunocytochemistry
8-25 µg/mL
Immersion fixed NTera-2 human testicular embryonic carcinoma cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Stella/Dppa3

Stella, also known as Pgc7 or Dppa3, is expressed in primordial germ cells at the time of their emergence in gastrulating embryos. Stella may be an important maternal factor in the cleavage stages of mouse embryogenesis and is required for germ cell specification.

Long Name
Developmental Pluripotency-associated Protein 3
Entrez Gene IDs
359787 (Human); 73708 (Mouse)
Alternate Names
developmental pluripotency associated 3; DPPA3 developmental pluripotency associated 3; Dppa3; Stella

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Citations for Mouse Stella/Dppa3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Maternal Factor Dppa3 Activates 2C-Like Genes and Depresses DNA Methylation in Mouse Embryonic Stem Cells
    Authors: Chuanyu Zhang, Hang Wen, Siying Liu, Enze Fu, Lu Yu, Shang Chen et al.
    Frontiers in Cell and Developmental Biology
  2. Expression of Genes Related to Germ Cell Lineage and Pluripotency in Single Cells and Colonies of Human Adult Germ Stem Cells
    Authors: Sabine Conrad, Hossein Azizi, Maryam Hatami, Mikael Kubista, Michael Bonin, Jörg Hennenlotter et al.
    Stem Cells International
  3. Generation of pluripotent stem cells from adult human testis.
    Authors: Conrad S, Renninger M, Hennenlotter J, Wiesner T, Just L, Bonin M, Aicher W, Buhring HJ, Mattheus U, Mack A, Wagner HJ, Minger S, Matzkies M, Reppel M, Hescheler J, Sievert KD, Stenzl A, Skutella T
    Nature, 2008-10-08;456(7220):344-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

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