Mouse Stella/Dppa3 Antibody

Name: Mouse Stella/Dppa3 Antibody
Brand: R&D Systems
SKU: AF2566
Rating: 4 (1 reviews)

Catalog #: AF2566
5 Citations 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF2566
AF2566-SP

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All Stella/Dppa3 Antibodies

Product Details

Citations (5)

FAQs

Supplemental Products

Reviews (1)

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Stella/Dppa3 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Stella/Dppa3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross‑reactivity with recombinant human Stella is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant mouse Stella/Dppa3
Met1-Asn150
Accession # Q8QZY3

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse Stella/Dppa3

Immunohistochemistry

1-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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Stella/Dppa3 in mouse ovary tissue. Stella/Dppa3 was detected in perfusion fixed frozen sections of mouse ovary tissue using Goat Anti-Mouse Stella/Dppa3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2566) at 1 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (brown; Catalog # VC004) and counterstained with hematoxylin (blue). Specific staining was localized to oocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Stella/Dppa3

Stella, also known as Dppa3 (Developmental pluripotency-associated 3) and PGC7 (primordial germ cell 7) is expressed in primordial germ cells, oocytes, preimplantation embryos, and pluripotent cells. Although the fuction of Stella is not known, the expression pattern of the protein suggests a role in the maintenance of cell pluripotency.

Long Name

Developmental Pluripotency-associated Protein 3

Entrez Gene IDs

359787 (Human); 73708 (Mouse)

Alternate Names

developmental pluripotency associated 3; DPPA3 developmental pluripotency associated 3; Dppa3; Stella

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Mouse Stella/Dppa3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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