Mouse TACI/TNFRSF13B PE-conjugated Antibody

Catalog #: FAB1041P
5 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
FAB1041P

Detection of TACI/TNFRSF13B in Mouse Splenocytes by Flow Cytometry.

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Citations (5)

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Mouse TACI/TNFRSF13B PE-conjugated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse TACI/TNFRSF13B in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant human (rh) TACI or rhBCMA is observed.

Source

Monoclonal Rat IgG_2A Clone # 166010

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse TACI/TNFRSF13B
Phe5-Thr129
Accession # Q9ET35

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of TACI/TNFRSF13B in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse B220/CD45R Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB1217F) and either (A) Rat Anti-Mouse TACI/TNFRSF13B PE-conjugated Monoclonal Antibody (Catalog # FAB1041P) or (B) Rat IgG_2APhycoerythrin Isotype Control (Catalog # IC006P). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: TACI/TNFRSF13B

TACI, Transmembrane Activator and CAML-Interactor, is a member of the TNF receptor superfamily and has been designated TNFRSF13B. TACI is a type III membrane protein with an extracellular N-terminus in the absence of a cleaved signal sequence. The extracellular region of TACI contains two cysteine-rich domains. Within the TNFRSF, it shares the highest homology with B cell Maturation Factor (BCMA). TACI and BCMA have both been shown to bind APRIL and BAFF, members of the TNF ligand superfamily. TACI is expressed on the cell surface of B cells and activated, but not resting, T cells. Analogous to BCMA, data suggests that TACI may play an important role in B cell development, function and regulation. Mature mouse TACI is a 249 amino acid (aa) protein consisting of a 129 aa extracellular domain, a 20 aa transmembrane domain, and a 100 aa intracellular domain. Mouse and human TACI share 54% aa identity.

Long Name

Transmembrane Activator and CAML Interactor/Tumor Necrosis Factor Receptor Superfamily Member 13B

Entrez Gene IDs

23495 (Human); 57916 (Mouse); 102126451 (Cynomolgus Monkey)

Alternate Names

CD267 antigen; CD267; CVID; CVID2; FLJ39942; MGC133214; TACI; TACIMGC39952; TNFRSF13B; TNFRSF14B; Transmembrane activator and CAML interactor; tumor necrosis factor receptor 13B; tumor necrosis factor receptor superfamily member 13B; tumor necrosis factor receptor superfamily, member 13B

Product Datasheets

Product Datasheet

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Related Research Areas

Citations for Mouse TACI/TNFRSF13B PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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Impaired B cell receptor signaling is responsible for reduced TACI expression and function in X-linked immunodeficient mice.
Authors: Uslu K, Coleman A, Allman W, Katsenelson N, Bram R, Alugupalli K, Akkoyunlu M
J Immunol, 2014-03-19;192(8):3582-95.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
The TACI receptor regulates T-cell-independent marginal zone B cell responses through innate activation-induced cell death.
Authors: Figgett W, Fairfax K, Vincent F, Le Page M, Katik I, Deliyanti D, Quah P, Verma P, Grumont R, Gerondakis S, Hertzog P, O'Reilly L, Strasser A, Mackay F
Immunity, 2013-09-05;39(3):573-83.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
FcgammaRIIB signals inhibit BLyS signaling and BCR-mediated BLyS receptor up-regulation.
Authors: Crowley JE, Stadanlick JE, Cambier JC, Cancro MP
Blood, 2008-09-12;113(7):1464-73.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
Deficient TACI expression on B lymphocytes of newborn mice leads to defective Ig secretion in response to BAFF or APRIL.
Authors: Kanswal S, Katsenelson N, Selvapandiyan A, Bram RJ, Akkoyunlu M
J. Immunol., 2008-07-15;181(2):976-90.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
APRIL facilitates viral-induced erythroleukemia but is dispensable for T cell immunity and lymphomagenesis.
Authors: Hardenberg G, Fernandez L, Hendriks J, Chebli K, Jacquet C, Sitbon M, Hahne M, Medema JP
J. Leukoc. Biol., 2008-05-15;84(2):380-8.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry