Mouse TDRD1 Antibody

Catalog #: MAB6296
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB6296
MAB6296-SP

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse TDRD1 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse TDRD1 in direct ELISAs. In direct ELISAs, 100% cross-reactivity with recombinant human TDRD1 is observed.

Source

Monoclonal Rat IgG_2A Clone # 739206

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant mouse TDRD1
Asn831-Lys960
Accession # Q99MV1

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Immunohistochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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TDRD1 in Mouse Testis. TDRD1 was detected in perfusion fixed frozen sections of mouse testis using Rat Anti-Mouse TDRD1 Monoclonal Antibody (Catalog # MAB6296) at 25 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to spermatogonia. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TDRD1

TDRD1 (Tudor domain containing protein 1; also MTR-1) is a 130-145 kDa member of the Tudor family of proteins. It is expressed in spermatocytes and spermatids, and forms part of an RNA-silencing complex that regulates gene expression. TDRD1, through its Tudor and MYND domains, binds methylated PIWI (P-element-induced wimpy testis) protein and PIWI-interacting RNA loading factor, respectively. This serves to promote the recruitment of germline-specific small RNAs into a PIWI ribonucleoprotein complex. Mouse TDRD1 is 1172 aa in length. It contains one MYND-type Zn finger region (aa 163-199), and four methyl-binding Tudor domains (aa 307-1032). Over aa 831-960, mouse TDRD1 shares 96% and 66% aa identity with rat and human TDRD1, respectively.

Long Name

Tudor Domain Containing 1

Entrez Gene IDs

56165 (Human); 83561 (Mouse); 292129 (Rat)

Alternate Names

CT41.1; CT41.1Cancer/testis antigen 41.1; FLJ21082; TDRD1; tudor domain containing 1; tudor domain containing protein 1; tudor domain-containing protein 1

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Primordial and Mature Germ Cells

Citation for Mouse TDRD1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1