Mouse TFPI-2 Antibody

Catalog #: AF4045 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF4045
AF4045-SP

All TFPI-2 Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse TFPI-2 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse TFPI-2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross‑reactivity with recombiannt human (rh) TFPI-2, rhTFPI-1, and recombinant mouse TFPI-1 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse TFPI-2
Leu23-Lys211
Accession # O35536

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse TFPI‑2

Immunoprecipitation

25 µg/mL

Conditioned cell culture medium spiked with Recombinant Mouse TFPI‑2, see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TFPI-2

TFPI-2 (tissue factor pathway inhibitor-2; also placental protein 5/PP5) is a secreted, presumably 27‑34 kDa glycoprotein member of the Kunitz domain-containing proteinase inhibitor family. In mouse, it is principally produced by endothelium, hepatocytes and synctiotrophoblasts, and serves as an inhibitor of multiple serine proteinases. Mature mouse TFPI-2 is 208 amino acids (aa) in length. It contains three Kunitz inhibitor domains, the most N-terminal of which (aa 36‑86) is crucial to inhibitor activity. Over aa 23‑211, mouse TFPI-2 shares 86% and 58% aa sequence identity with rat and human TFPI-2, respectively.

Long Name

Tissue Factor Pathway Inhibitor 2

Entrez Gene IDs

7980 (Human)

Alternate Names

Placental protein 5; PP5; PP5FLJ21164; REF-1; TFPI2; TFPI-2; TFPI-2REF1; tissue factor pathway inhibitor 2