Mouse TGF-beta 2 Antibody Summary
Ala303-Ser414
Accession # P27090
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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TGF‑ beta 2 Inhibition of IL‑4-dependent Cell Proliferation and Neutralization by Mouse TGF‑ beta 2 Antibody. Recombinant Mouse TGF-beta 2 (Catalog # 7346-B2) inhibits Recombinant Mouse IL-4 (Catalog # 404-ML) induced proliferation in the HT-2 mouse T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Mouse IL-4 (7.5 ng/mL) activity elicited by Recombinant Mouse TGF-beta 2 (5 ng/mL) is neutralized (green line) by increasing concen-trations of Rat Anti-Mouse TGF-beta 2 Monoclonal Antibody (Catalog # MAB7346). The ND50 is typically 0.15-0.75 ug/mL.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TGF-beta 2
TGF-beta 2 (transforming growth factor beta 2) is one of three closely related mammalian members of the large TGF-beta superfamily that share a characteristic cysteine knot structure. TGF-beta 1, -2 and -3 are highly pleiotropic cytokines proposed to act as cellular switches that regulate processes such as immune function, proliferation and epithelial-mesenchymal transition. Each TGF-beta isoform has some non-redundant functions; for TGF-beta 2, mice with targeted deletion show defects in development of cardiac, lung, craniofacial, limb, eye, ear and urogenital systems. Mouse TGF-beta 2 cDNA encodes a 414 amino acid (aa) precursor that contains a 19 aa signal peptide and a 395 aa proprotein. A furin-like convertase processes the proprotein to generate an N-terminal 283 aa latency-associated peptide (LAP) and a C-terminal 112 aa mature TGF- beta 2. Disulfide-linked homodimers of LAP and TGF-beta 2 remain non-covalently associated after secretion, forming the small latent TGF-beta 2 complex. Covalent linkage of LAP to one of three latent TGF-beta binding proteins (LTBPs) creates a large latent complex that may interact with the extracellular matrix. TGF-beta is activated from latency by pathways that include actions of the protease plasmin, matrix metalloproteases, thrombospondin 1 and a subset of integrins. Mature mouse TGF-beta 2 shares 100% aa identity with rat TGF-beta 2, and 97% aa identity with human, porcine, canine, equine and bovine TGF-beta 2. It demonstrates cross-species activity. In most cells, TGF-beta 2 signaling begins with binding to a complex of the accessory receptor betaglycan (also known as TGF-beta RIII) and a type II ser/thr kinase receptor termed TGF-beta RII, which then phosphorylates and activates another ser/thr kinase receptor, TGF-beta RI (also called activin receptor-like kinase (ALK) -5), or alternatively, ALK-1. The whole complex phosphorylates and activates Smad proteins that regulate transcription. In bone -related cells, however, TGF-beta 2 also signals through TGF-beta RIIB (a splice variant of TGF-beta RII), independently of TGF-beta RIII. Use of other signaling pathways that are Smad-independent allows for disparate actions observed in response to TGF-beta in different contexts.
Product Datasheets
Citations for Mouse TGF-beta 2 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 5
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Elevated TGF β2 serum levels in Emery-Dreifuss muscular dystrophy: implications for myocyte and tenocyte differentiation and fibrogenic processes
Authors: P Bernasconi, N Carboni, G Ricci, G Siciliano, L Politano, L Maggi, T Mongini, L Vercelli, C Rodolico, E Biagini, G Boriani, L Ruggiero, L Santoro, E Schena, S Prencipe, C Evangelist, E Pegoraro, L Morandi, M Columbaro, C Lanzuolo, P Sabatelli, P Cavalcante, C Cappellett, G Bonne, A Muchir, G Lattanzi
Nucleus, 2018-01-01;0(0):1-24.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization -
CYP4A in tumor-associated macrophages promotes pre-metastatic niche formation and metastasis
Authors: XW Chen, TJ Yu, J Zhang, Y Li, HL Chen, GF Yang, W Yu, YZ Liu, XX Liu, CF Duan, HL Tang, M Qiu, CL Wang, H Zheng, J Yue, AM Guo, J Yang
Oncogene, 2017-05-08;0(0):.
Species: Mouse
Sample Types: Cell Culture Supernates
Applications: Functional Assay -
Immunomodulatory cross-talk between conjunctival goblet cells and dendritic cells.
Authors: Contreras-Ruiz L, Masli S
PLoS ONE, 2015-03-20;10(3):e0120284.
Species: Mouse
Sample Types: Cell Culture Supernates
Applications: Neutralization -
Neutrophils increase or reduce parasite burden in Trypanosoma cruzi-infected macrophages, depending on host strain: role of neutrophil elastase.
Authors: Luna-Gomes T, Filardy A, Rocha J, Decote-Ricardo D, LaRocque-de-Freitas I, Morrot A, Bozza P, Castro-Faria-Neto H, DosReis G, Nunes M, Freire-de-Lima C
PLoS ONE, 2014-03-05;9(3):e90582.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Endothelial lineage differentiation from induced pluripotent stem cells is regulated by microRNA-21 and transforming growth factor beta2 (TGF-beta2) pathways.
Authors: Di Bernardini E, Campagnolo P, Margariti A, Zampetaki A, Karamariti E, Hu Y, Xu Q
J Biol Chem, 2013-12-19;289(6):3383-93.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization
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