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Mouse TIM-1/KIM-1/HAVCR Quantikine ELISA Kit

Catalog #: MKM100
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Mouse KIM-1 ELISA Standard Curve
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Summary Sample Values Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Mouse TIM-1/KIM-1/HAVCR Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
3.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL), Urine (10 uL)
Sensitivity
2.18 pg/mL
Assay Range
7.8 - 500 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine)
Specificity
Natural and recombinant mouse TIM-1. The antibody components of this kit were raised against the short splice form of mouse TIM-1 (isoform 2) which has a 23 amino acid deletion following Pro182. This immunoassay detects both the short and long forms of mouse TIM-1.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Sample Values

Serum/Plasma/Urine - Samples were evaluated for the presence of mouse TIM-1 in this assay

NSA StrainMean of Detectable (pg/mL)% DetectableRange (pg/mL)
Serum (n=20)17.290ND-42.5
EDTA plasma (n=20)25.61007.87-174
Heparin plasma (n=20)18.095ND-42.8
Urine (n=20)2248100456-8048
ND=Non-Detectable

Serum samples from two Balb/c strain mice were evaluated in this assay and measured 27.5 pg/mL and 26.9 pg/mL. Additionally, serum samples from two C57 strain mice were evaluated in this assay and measured 20.4 pg/mL and 14.4 pg/mL. 

Urine samples from two Balb/c strain mice and one C57 strain mouse were evaluated in this assay and measured 143,000 pg/mL, 159,000 pg/mL, and 726 pg/mL respectively.

Cell Culture Supernates - Kidneys from mice were removed, rinsed in 1X PBS, and kept on ice in 1X PBS. Kidneys were then homogenized using a tissue homogenizer and seeded into media containing RPMI 1640 supplemented with 10% fetal bovine serum, 2 mM L-glutamine, 100 U/mL penicillin, and 100 mg/mL streptomycin sulfate. Cells were cultured for the times indicated. Aliquots of the cell culture supernates were removed and assayed for levels of mouse TIM-1.

Tissue Type(pg/mL)
NSA mouse kidney (1 day)1006
NSA mouse kidney (3 days)756
Balb/c mouse kidney (2 days)1679
C57 mouse kidney (2 days)24




Product Summary

The Quantikine Mouse TIM-1 Immunoassay is a 3.5 hour solid phase ELISA designed to measure mouse TIM-1 in cell culture supernates, serum, plasma, and urine. It contains NS0-expressed recombinant mouse TIM-1 and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant factor accurately. Results obtained using natural mouse TIM-1 showed dose-response curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse TIM-1.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 24.2 61.8 207 23.2 60 190
Standard Deviation 0.8 1.6 6.8 2.5 4.2 13.2
CV% 3.3 2.6 3.3 10.8 7 6.9

Recovery

The recovery of TIM-1 spiked into various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 100 91-106
EDTA Plasma (n=4) 89 88-91
Heparin Plasma (n=4) 87 86-89
Serum (n=4) 95 93-97
Urine (n=4) 102 94-115

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of mouse TIM-1 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Mouse KIM-1 ELISA Linearity

Scientific Data

N/A Mouse KIM-1 ELISA Standard Curve View Larger

Mouse KIM-1 ELISA Standard Curve

Product Datasheets

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: TIM-1/KIM-1/HAVCR

TIM-1 (T cell immunoglobulin and mucin domain 1), also known as KIM-1 and HAVcr1, is expressed on many immune cell types and epithelial cells. It binds to phosphatidylserine (PS), LMIR5/CD300b, TIM-1 (homophilic), TIM-4, IgA, and the glycoproteins of a number of enveloped viruses. Its interaction with PS enables TIM-1 to mediate the phagocytosis and clearance of apoptotic cells. TIM-1 signaling costimulates T cell activation and enhances Th2 cytokine production. TIM-1 serves as a cellular entry receptor for hepatitis A virus, Ebolavirus and Marburgvirus. Polymorphisms are associated with susceptibility to atopy, autoimmunity, and severe hepatitis A virus infection in humans. A soluble form of TIM-1 is elevated in the urine during nephropathy.

Long Name:
T Cell Immunoglobulin Mucin-1
Entrez Gene IDs:
26762 (Human); 171283 (Mouse); 286934 (Rat); 102141332 (Cynomolgus Monkey)
Alternate Names:
CD365; HAVCR1; HAVCR-1; HAVCRT cell immunoglobin domain and mucin domain protein 1; hepatitis A virus cellular receptor 1; Kidney injury molecule 1; KIM1; KIM-1; T-cell immunoglobulin and mucin domain-containing protein 1; TIM1; TIM-1; TIM-1TIM; TIM1TIMD-1; TIMD1T-cell membrane protein 1
&#9888; WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

The conjugate must remain at 2-8 °C during use. Bring all other reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 50 µL Assay Diluent
  3.   Add 50 µL of Assay Diluent to each well.
    4. 50 µL Standard, Control, or Sample
  4.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
  5.   Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.
    6. 100 µL cold Conjugate
  6.   Add 100 µL of cold Conjugate to each well. Cover with a new plate sealer, and incubate at 2-8 °C for 1 hour on the benchtop.
  7.   Aspirate and wash 5 times.
    8. 100 µL Substrate Solution
  8.   Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.
    9. 100 µL Stop Solution
  9.   Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Mouse TIM-1/KIM-1/HAVCR Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

25 Citations: Showing 1 - 10
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  1. Pyruvate kinase M2 regulates mitochondrial homeostasis in cisplatin-induced acute kidney injury
    Authors: Xie, W;He, Q;Zhang, Y;Xu, X;Wen, P;Cao, H;Zhou, Y;Luo, J;Yang, J;Jiang, L;
    Cell death & disease
    Species: Mouse
    Sample Types: Urine
  2. Mesenchymal Stem Cells Delivered Locally to Ischemia-Reperfused Kidneys via Injectable Hyaluronic Acid Hydrogels Decrease Extracellular Matrix Remodeling 1 Month after Injury in Male Mice
    Authors: Han, DS;Erickson, C;Hansen, KC;Kirkbride-Romeo, L;He, Z;Rodell, CB;Soranno, DE;
    Cells
    Species: Mouse
    Sample Types: Urine
  3. The VEGF Inhibitor Soluble Fms-like Tyrosine Kinase 1 Does Not Promote AKI-to-CKD Transition
    Authors: CCL van Aanhol, A Koudijs, KL Dijkstra, R Wolterbeek, JA Bruijn, C van Kooten, HJ Baelde
    International Journal of Molecular Sciences, 2022-08-26;23(17):.
    Species: Mouse
    Sample Types: Serum
  4. Immune-mediated tubule atrophy promotes acute kidney injury to chronic kidney disease transition
    Authors: L Xu, J Guo, DG Moledina, LG Cantley
    Nature Communications, 2022-08-19;13(1):4892.
    Species: Mouse
    Sample Types: Serum
  5. Effects of Suramin on Polycystic Kidney Disease in a Mouse Model of Polycystin-1 Deficiency
    Authors: MY Chang, SH Hsu, LY Ma, LF Chou, CC Hung, YC Tian, CW Yang
    International Journal of Molecular Sciences, 2022-07-31;23(15):.
    Species: Mouse
    Sample Types: Plasma
  6. Snapshots of nascent RNA reveal cell- and stimulus- specific responses to acute kidney injury
    Authors: TH Shen, J Stauber, K Xu, A Jacunski, N Paragas, M Callahan, R Banlengchi, AD Levitman, B Desanti de, A Beenken, MS Grau, E Mathieu, Q Zhang, Y Li, T Gopal, N Askanase, S Arumugam, S Mohan, PI Good, JS Stevens, F Lin, SK Sia, CS Lin, V D'Agati, K Kiryluk, NP Tatonetti, J Barasch
    JCI Insight, 2022-03-22;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  7. Juzentaihoto improves adenine-induced chronic renal failure in BALB/c mice via suppression of renal fibrosis and inflammation
    Authors: S Ito, E Manabe, Y Dai, M Ishihara, T Tsujino
    Journal of pharmacological sciences, 2021-10-25;148(1):172-178.
    Species: Mouse
    Sample Types: Urine
  8. DNA-Aptamer Raised against Receptor for Advanced Glycation End Products Improves Survival Rate in Septic Mice
    Authors: Y Koga, A Sotokawauc, Y Higashimot, Y Nishino, N Hashizume, T Kakuma, J Akiba, Y Tanaka, T Matsui, M Yagi, SI Yamagishi
    Oxidative Medicine and Cellular Longevity, 2021-08-07;2021(0):9932311.
    Species: Mouse
    Sample Types: Urine
  9. MicroRNA?93 inhibits the apoptosis and inflammatory response of tubular epithelial cells via the PTEN/AKT/mTOR pathway in acute kidney injury
    Authors: Y Zhan, M Zhu, S Liu, J Lu, Z Ni, H Cai, W Zhang
    Molecular Medicine Reports, 2021-07-23;24(3):.
    Species: Mouse
    Sample Types: Serum
  10. CO-Releasing Molecule-2 Prevents Acute Kidney Injury through Suppression of ROS-Fyn-ER Stress Signaling in Mouse Model
    Authors: MJ Uddin, J Jeong, ES Pak, H Ha
    Oxidative Medicine and Cellular Longevity, 2021-07-06;2021(0):9947772.
    Species: Mouse
    Sample Types: Plasma
  11. Potential Biomarkers in Diagnosis of Renal Acanthamoebiasis
    Authors: K Kot, P Kupnicka, O Witulska, A Czepan, NA ?anocha-Ar, AA ?anocha, DI Kosik-Boga
    International Journal of Molecular Sciences, 2021-06-19;22(12):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  12. Angiotensin AT2 Receptor is Anti-inflammatory and Reno-Protective in Lipopolysaccharide Mice Model: Role of IL-10
    Authors: N Fatima, S Patel, T Hussain
    Frontiers in Pharmacology, 2021-04-15;12(0):600163.
    Species: Mouse
    Sample Types: Tissue Homogenates
  13. Inhibition of Src Family Kinases Ameliorates LPS-Induced Acute Kidney Injury and Mitochondrial Dysfunction in Mice
    Authors: ES Pak, MJ Uddin, H Ha
    Int J Mol Sci, 2020-11-03;21(21):.
    Species: Mouse
    Sample Types: Plasma
  14. Proximal Tubule mTORC1 Is a Central Player in the Pathophysiology of Diabetic Nephropathy and Its Correction by SGLT2 Inhibitors
    Authors: A Kogot-Levi, L Hinden, Y Riahi, T Israeli, B Tirosh, E Cerasi, EB Mizrachi, J Tam, O Mosenzon, G Leibowitz
    Cell Rep, 2020-07-28;32(4):107954.
    Species: Mouse
    Sample Types: Urine
  15. Pro-inflammatory P2Y14 receptor inhibition protects against ischemic acute kidney injury in mice
    Authors: MA Battistone, AC Mendelsohn, RG Spallanzan, AS Allegretti, RN Liberman, J Sesma, S Kalim, SM Wall, JV Bonventre, ER Lazarowski, D Brown, S Breton
    J. Clin. Invest., 2020-07-01;0(0):.
    Species: Mouse
    Sample Types: Serum
  16. Distinct Functional Requirements for Podocalyxin in Immature and Mature Podocytes Reveal Mechanisms of Human Kidney Disease
    Authors: I Refaeli, MR Hughes, AK Wong, MLZ Bissonnett, CD Roskelley, A Wayne Vogl, SJ Barbour, BS Freedman, KM McNagny
    Sci Rep, 2020-06-10;10(1):9419.
    Species: Mouse
    Sample Types: Urine
  17. The C5a/C5aR2 axis promotes renal inflammation and tissue damage
    Authors: T Zhang, KY Wu, N Ma, LL Wei, M Garstka, W Zhou, K Li
    JCI Insight, 2020-04-09;5(7):.
    Species: Mouse
    Sample Types: Tissue Lysates
  18. The iron chelator Deferasirox causes severe mitochondrial swelling without depolarization due to a specific effect on inner membrane permeability
    Authors: EM Gottwald, CD Schuh, P Drücker, D Haenni, A Pearson, S Ghazi, M Bugarski, M Polesel, M Duss, EM Landau, A Kaech, U Ziegler, AKM Lundby, C Lundby, PS Dittrich, AM Hall
    Sci Rep, 2020-01-31;10(1):1577.
    Species: Mouse
    Sample Types: Urine
  19. Using Collaborative Cross Mouse Population to Fill Data Gaps in Risk Assessment: A Case Study of Population-Based Analysis of Toxicokinetics and Kidney Toxicodynamics of Tetrachloroethylene
    Authors: YS Luo, JA Cichocki, NH Hsieh, L Lewis, FA Wright, DW Threadgill, WA Chiu, I Rusyn
    Environ. Health Perspect., 2019-06-27;127(6):67011.
    Species: Mouse
    Sample Types: Tissue Culture Supernates
  20. Immunoglobulin light chains generate proinflammatory and profibrotic kidney injury
    Authors: WZ Ying, X Li, S Rangarajan, W Feng, LM Curtis, PW Sanders
    J. Clin. Invest., 2019-06-17;129(7):2792-2806.
    Species: Mouse
    Sample Types: Urine
  21. Uncoupling Protein 2 Increases Blood Pressure in DJ -1 Knockout Mice
    Authors: C De Miguel, WC Hamrick, R Sedaka, S Jagarlamud, LD Asico, PA Jose, S Cuevas
    J Am Heart Assoc, 2019-05-07;8(9):e011856.
    Species: Mouse
    Sample Types: Urine
  22. Disruption of renal arginine metabolism promotes kidney injury in hepatorenal syndrome
    Authors: ZV Varga, K Erdelyi, J Paloczi, R Cinar, ZK Zsengeller, T Jourdan, C Matyas, NT Balazs, A Guillot, X Xiang, A Mehal, G Hasko, IE Stillman, S Rosen, B Gao, G Kunos, P Pacher
    Hepatology, 2018-10-01;0(0):.
    Species: Mouse
    Sample Types: Serum
  23. Ameliorative Effect of Daidzein on Cisplatin-Induced Nephrotoxicity in Mice via Modulation of Inflammation, Oxidative Stress, and Cell Death
    Authors: H Meng, G Fu, J Shen, K Shen, Z Xu, Y Wang, B Jin, H Pan
    Oxid Med Cell Longev, 2017-08-02;2017(0):3140680.
    Species: Mouse
    Sample Types: Urine
  24. Human Alpha-1-Antitrypsin (hAAT) therapy reduces renal dysfunction and acute tubular necrosis in a murine model of bilateral kidney ischemia-reperfusion injury
    Authors: N Maicas, J van der Vl, J Bublitz, S Florquin, M Bakker-van, CA Dinarello, V Verweij, R Masereeuw, LA Joosten, LB Hilbrands
    PLoS ONE, 2017-02-24;12(2):e0168981.
    Species: Mouse
    Sample Types: Plasma
  25. Senescence marker protein-30/gluconolactonase deficiency exacerbates diabetic nephropathy through tubular injury in a mouse model of type 1 diabetes.
    Authors: Okada H, Senmaru T, Fukui M, Kondo Y, Ishigami A, Maruyama N, Obayashi H, Yamazaki M, Nakamura N, Hasegawa G
    J Diabetes Investig, 2014-07-07;6(1):35-43.
    Species: Mouse
    Sample Types: Urine

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Reviews for Mouse TIM-1/KIM-1/HAVCR Quantikine ELISA Kit

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Mouse TIM-1/KIM-1/HAVCR Quantikine ELISA Kit
By Anonymous on 02/10/2021
Sample Tested: Kidney tissue,Urine
Mouse TIM-1/KIM-1/HAVCR Quantikine ELISA Kit MKM100
Mouse TIM-1/KIM-1/HAVCR Quantikine ELISA Kit
By Wen Li on 11/13/2019
Sample Tested: Urine