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Mouse TIM-3 APC-conjugated Antibody

Catalog #: FAB1529A
4 Citations Datasheet / COA / SDS
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FAB1529A
Detection of TIM‑3 in RAW 264.7 Mouse Cell Line by Flow Cytometry.
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Citations (4)
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Mouse TIM-3 APC-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse TIM-3 in direct ELISAs and Western blots.
Source
Monoclonal Rat IgG2A Clone # 215008
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse TIM‑3
Arg20-Ala193
Accession # Q8VIM0
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of TIM-3 antibody in RAW 264.7 Mouse Cell Line antibody by Flow Cytometry. View Larger

Detection of TIM‑3 in RAW 264.7 Mouse Cell Line by Flow Cytometry. RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse TIM-3 APC-conjugated Monoclonal Antibody (Catalog # FAB1529A, filled histogram) or isotype control antibody (Catalog # IC006A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: TIM-3

TIM-3 (T cell immunoglobulin and mucin domain-3) is a 60 kDa member of the TIM family of immune regulating molecules. TIMs are type I transmembrane glycoproteins with one Ig-like V-type domain and a Ser/Thr-rich mucin stalk (1 - 3). There are three TIM genes in human and eight in mouse. Mature mouse TIM-3 consists of a 174 aa extracellular domain (ECD), a 21 aa transmembrane segment (TM), and a 67 aa cytoplasmic tail (4). Two alternately spliced isoforms have been reported in mouse which lack either the TM or both the TM and mucin regions (5, 6). Within the ECD, mouse TIM-3 shares 58% and 74% aa sequence identity with human and rat TIM-3, respectively. TIM-3 is specifically expressed on Th1 cells whereas TIM-1 and TIM-2 are expressed on TH2 cells. In chronic inflammation, autoimmune disorders, and some cancers, TIM-3 is upregulated on several other hematopoietic cell types and on hippocampal neurons (9 - 12). The glycosylated Ig domain of TIM-3 binds cell-associated galectin-9 which induces TIM-3 Tyr phosphorylation and proapoptotic signaling(10, 13). TIM-3 functions as a negative regulator of Th1 cell activity. Its blockade results in increased IFN-gamma production, Th1 cell proliferation, and cytotoxicity (5, 7, 12, 14). TIM-3 may play a role in regulatory T cell development, (7) inflammation, (15) and immune tolerance (5, 13, 14). Soluble mouse TIM-3 has been shown to inhibit anti-tumor effector T cell responses and to enhance autoimmune reactions (6, 7).

References
  1. Anderson, A.C. and D.E. Anderson (2006) Curr. Opin. Immunol. 18:665.
  2. Mariat, C. et al. (2005) Phil. Trans. R. Soc. B 360:1681.
  3. Meyers, J.H. et al. (2005) Trends Mol. Med. 11:362.
  4. Monney, L. et al. (2002) Nature 415:536.
  5. Sabatos, C.A. et al. (2003) Nat. Immunol. 4:1102.
  6. Geng, H. et al. (2006) J. Immunol. 176:1411.
  7. Sanchez-Fueyo, A. et al. (2003) Nat. Immunol. 4:1093.
  8. Khademi, M. et al. (2004) J. Immunol. 172:7169.
  9. Wiener, Z. et al. (2007) J. Invest. Dermatol. 127:906.
  10. van de Weyer, P.S. et al. (2006) Biochem. Biophys. Res. Commun. 351:571.
  11. Gielen, A.W. et al. (2005) J. Neuroimmunol. 164:93.
  12. Oikawa, T. et al. (2006) J. Immunol. 177:4281.
  13. Zhu, C. et al. (2005) Nat. Immunol. 6:1245.
  14. Koguchi, K. et al. (2006) J. Exp. Med. 203:1413.
  15. Frisancho-Kiss, S. et al. (2006) J. Immunol. 176:6411.
Long Name
T Cell Immunoglobulin Mucin-3
Entrez Gene IDs
84868 (Human); 171285 (Mouse); 363578 (Rat); 479318 (Canine); 102141722 (Cynomolgus Monkey)
Alternate Names
CD366; FLJ14428; HAVCR2; HAVcr-2; hepatitis A virus cellular receptor 2; kidney injury molecule-3; KIM-3; SPTCL; T cell immunoglobulin mucin 3; T cell immunoglobulin mucin-3; T-cell immunoglobulin and mucin domain-containing protein 3; TIM 3; TIM3 T-cell membrane protein 3; TIM3; TIM-3; TIMD3; TIMD-3; TIMD3KIM-3

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Citations for Mouse TIM-3 APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. CD8+ T Cell Priming in Established Chronic Viral Infection Preferentially Directs Differentiation of Memory-like Cells for Sustained Immunity
    Authors: Laura M. Snell, Bethany L. MacLeod, Jaclyn C. Law, Ivan Osokine, Heidi J. Elsaesser, Kebria Hezaveh et al.
    Immunity
  2. Predicting immunogenic tumour mutations by combining mass spectrometry and exome sequencing.
    Authors: Yadav M, Jhunjhunwala S, Phung Q, Lupardus P, Tanguay J, Bumbaca S, Franci C, Cheung T, Fritsche J, Weinschenk T, Modrusan Z, Mellman I, Lill J, Delamarre L
    Nature, 2014-11-27;515(7528):572-6.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Highly pathological influenza A virus infection is associated with augmented expression of PD-1 by functionally compromised virus-specific CD8+ T cells.
    Authors: Rutigliano, John A, Sharma, Shalini, Morris, Melissa, Oguin, Thomas H, McClaren, Jennifer, Doherty, Peter C, Thomas, Paul G
    J Virol, 2013-11-20;88(3):1636-51.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Flow Cytometry
  4. Role of Tim-3/galectin-9 inhibitory interaction in viral-induced immunopathology: shifting the balance toward regulators.
    Authors: Sehrawat S, Suryawanshi A, Hirashima M, Rouse BT
    J. Immunol., 2009-03-01;182(5):3191-201.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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