Mouse TIM-4 Antibody

Catalog #: AF2826
4 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF2826
AF2826-SP

All TIM-4 Antibodies

Product Details

Citations (4)

Supplemental Products

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse TIM-4 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse TIM-4 in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross‑reactivity with recombinant human (rh) TIM-1, recombinant mouse (rm) TIM-2, rmTIM-3, rhTIM-4, rmTIM-5, and rmTIM-6 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse TIM-4
Ala22-Thr279
Accession # Q6U7R4

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse TIM‑4 (Catalog # 2826-TI)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TIM-4

TIM-4 (T cell; immunoglobulin; mucin-4), also known as SMUCKLER, is a 60 kDa member of the TIM family of immune regulating proteins. TIMs are type I transmembrane proteins with one Ig-like V domain and one Ser/Thr-rich mucin domain (1 - 3). The mouse TIM-4 cDNA encodes a 343 amino acid (aa) precursor that includes a 22 aa signal sequence, a 257 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 43 aa cytoplasmic tail (4). Structurally, TIM-4 is distinguished from other TIMs by the presence of an RGD motif in its Ig domain and the lack of a site for tyrosine phosphorylation in its cytoplasmic tail. The mucin domain in TIM-4 is larger than in TIM-1, -2, or -3. Within the ECD, mouse TIM-4 shares 27 - 33% aa sequence identity with mouse TIM-1, -2, and -3. The ECD of mouse TIM-4 shares 45% aa sequence identity with that of human and rat TIM-4. TIM-4 is expressed by macrophages and mature dendritic cells but not by lymphocytes (4, 5). TIM-4 binds specifically to TIM-1 which is also the cellular receptor for hepatitis A virus and has been implicated in the development of asthma (5 ‑ 7). Among hematopoietic cells, TIM-1 is expressed on activated B and T cells, preferentially in the Th2 subset of CD4⁺ T cells (5, 8). The interaction of TIM-4 with TIM-1 induces co-stimulatory and hyperproliferative signals in T cells (5).

References

Kuchroo, V.K. et al. (2003) Nat. Rev. Immunol. 3:454.
Mariat, C. et al. (2005) Phil. Trans. R. Soc. B 360:1681.
Meyers, J.H. et al. (2005) Trends Mol. Med. 11:362.
Shakhov, A.N. et al. (2004) Eur. J. Immunol. 34:494.
Meyers, J.H. et al. (2005) Nat. Immunol. 6:455.
Feigelstock, D. et al. (1998) J. Virol. 72:6621.
McIntire, J.J. et al. (2001) Nat. Immunol. 2:1109.
Khademi, M. et al. (2004) J. Immunol. 172:7169.

Long Name

T Cell Immunoglobulin Mucin-4

Entrez Gene IDs

91937 (Human); 276891 (Mouse); 102140672 (Cynomolgus Monkey)

Alternate Names

FLJ27515; Smuckler; T-cell immunoglobulin and mucin domain containing 4; T-cell immunoglobulin and mucin domain-containing protein 4; T-cell membrane protein 4; TIM4; TIM-4; TIMD4; TIMD-4

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Mouse TIM-4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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IL-4/IL-13 polarization of macrophages enhances Ebola virus glycoprotein-dependent infection
Authors: KJ Rogers, B Brunton, L Mallinger, D Bohan, KM Sevcik, J Chen, N Ruggio, W Maury
PLoS Negl Trop Dis, 2019-12-11;13(12):e0007819.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
Mouse macrophages show different requirements for phosphatidylserine receptor Tim4 in efferocytosis
Authors: Y Yanagihash, K Segawa, R Maeda, YI Nabeshima, S Nagata
Proc. Natl. Acad. Sci. U.S.A., 2017-08-02;0(0):.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot
T cell Ig domain and mucin domain 1 engagement on invariant NKT cells in the presence of TCR stimulation enhances IL-4 production but inhibits IFN-gamma production.
Authors: Kim HS, Kim HS, Lee CW, Chung DH
J. Immunol., 2010-03-10;184(8):4095-106.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot
TIM-1 and TIM-3 enhancement of Th2 cytokine production by mast cells.
Authors: Nakae S, Iikura M, Suto H, Akiba H, Umetsu DT, DeKruyff RH, Saito H, Galli SJ
Blood, 2007-07-09;110(7):2565-8.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot