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Mouse TLR4 APC-conjugated Antibody

Catalog #: FAB2759A
1 Citation Datasheet / COA / SDS

Discontinued Product

FAB2759A has been discontinued.
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Detection of TLR4 in LPS-activated RAW 264.7 Mouse Cell Line by Flow Cytometry.
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Mouse TLR4 APC-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse TLR4 in direct ELISAs and Western blots. In direct ELISAs, does not cross-react with recombinant mouse TLR1, 2, 3, 6, or recombinant human TLR4.
Source
Monoclonal Rat IgG2A Clone # 267518
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse TLR4
Asn26-Lys629
Accession # Q9QUK6
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of TLR4 antibody in LPS-activated RAW 264.7 Mouse Cell Line antibody by Flow Cytometry. View Larger

Detection of TLR4 in LPS-activated RAW 264.7 Mouse Cell Line by Flow Cytometry. LPS-activated RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse TLR4 APC-conjugated Monoclonal Antibody (Catalog # FAB2759A, filled histogram) or isotype control antibody (Catalog # IC006A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: TLR4

TLR4 belongs to the IL-1 receptor/Toll-like receptor superfamily. It is expressed in placenta and peripheral blood leukocytes. TLR4 is the pattern recognition receptor for LPS.

Long Name
Toll-like Receptor 4
Entrez Gene IDs
7099 (Human); 21898 (Mouse); 29260 (Rat)
Alternate Names
ARMD10; CD_antigen: CD284; CD284 antigen; CD284; EC 3.2.2.6; EC:3.2.2.6; homolog of Drosophila toll; hToll; TLR4; TLR-4; toll like receptor 4 protein; TOLL; toll-like receptor 4

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Citation for Mouse TLR4 APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Protection against collagen-induced arthritis in mice afforded by the parasitic worm product, ES-62, is associated with restoration of the levels of interleukin-10-producing B cells and reduced plasma cell infiltration of the joints.
    Authors: Rodgers D, Pineda M, McGrath M, Al-Riyami L, Harnett W, Harnett M
    Immunology, 2014-03-01;141(3):457-66.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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