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Mouse TREM-1 Antibody

Catalog #: AF1187
8 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF1187
AF1187-SP
TREM‑1 in Mouse Splenocytes.
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Citations (8)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse TREM-1 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse TREM-1 in ELISAs and Western blots. In sandwich immunoassays, less than 0.2% cross‑reactivity with recombinant human (rh) TREM-1 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse TREM-1
Ala21-Ser202
Accession # Q9JKE2
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse TREM‑1 Fc Chimera (Catalog # 1187-TR)
Flow Cytometry
0.25 µg/106 cells
Mouse blood GR‑1+ granulocytes
Agonist Activity
Measured by its ability to stimulate TNF-alpha secretion by P388D1 mouse lymphoma cells (ATCC # TIB-63). Bouchon, A. et al. (2001) Nature, 410:1103 and Bouchon, A. et al. (2000) J. Immunology, 164:4991.
The ED50 for this effect is typically 1‑3 μg/mL.
 
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
5-15 µg/mL
See below

Mouse TREM-1 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
0.2-0.8 µg/mL 

Use in combination with:

Detection Reagent: Mouse TREM‑1 Biotinylated Antibody (Catalog # BAF1187)

Standard: Recombinant Mouse TREM-1 Fc Chimera Protein, CF (Catalog # 1187-TR)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry TREM-1 antibody in Mouse Splenocytes by Immunocytochemistry (ICC). View Larger

TREM‑1 in Mouse Splenocytes. TREM-1 was detected in immersion fixed mouse splenocytes treated with LPS (left panel) or untreated (right panel) using Goat Anti-Mouse TREM-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1187) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TREM-1

TREM-1 (Triggering Receptor Expressed on Myeloid cells) is a type I transmembrane protein having a single Ig-like domain. It associates with the adapter protein, DAP12, to deliver an activating signal. Several other TREM family members have been reported that are structurally similar but share less than 30% amino acid identity. TREM-1 is expressed on blood neutrophils and a subset of monocytes, and expression is up-regulated by bacterial LPS. Engagement of TREM-1 with a monoclonal antibody leads to expression of IL-8, MCP-1, and TNF-alpha suggesting that this receptor plays an important role in inflammatory responses. TREM-1 is expressed at high levels on neutrophils of patients with microbial sepsis and in mice with LPS-induced shock. Blockade of TREM-1 with a TREM-1/Fc fusion protein protected mice against LPS-induced shock.

References
  1. Bouchon, A. (2000) J. Immunol. 164:4991.
  2. Bouchon, A. (2001) Nature 410:1103.
  3. Nathan, C. and A. Ding (2001) Nature Med. 7:530.
Long Name
Triggering Receptor Expressed on Myeloid Cells 1
Entrez Gene IDs
54210 (Human); 58217 (Mouse)
Alternate Names
CD354 antigen; CD354; TREM1; TREM-1; TREM-1Triggering receptor expressed on monocytes 1; triggering receptor expressed on myeloid cells 1; triggering-receptor TREM1

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Citations for Mouse TREM-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. Triggering Receptor Expressed on Myeloid Cells-1 Agonist Regulates Intestinal Inflammation via Cd177+ Neutrophils
    Authors: Dong Hyuk Seo, Xiumei Che, Soochan Kim, Da Hye Kim, Hyun Woo Ma, Jae Hyeon Kim et al.
    Frontiers in Immunology
  2. Extracellular CIRP as an endogenous TREM-1 ligand to fuel inflammation in sepsis
    Authors: NL Denning, M Aziz, A Murao, SD Gurien, M Ochani, JM Prince, P Wang
    JCI Insight, 2020-03-12;0(0):.
  3. Enhancing protective microglial activities with a dual function TREM2 antibody to the stalk region
    Authors: K Schlepckow, KM Monroe, G Kleinberge, L Cantuti-Ca, S Parhizkar, D Xia, M Willem, G Werner, N Pettkus, B Brunner, A Sülzen, B Nuscher, H Hampel, X Xiang, R Feederle, S Tahirovic, JI Park, R Prorok, C Mahon, CC Liang, J Shi, DJ Kim, H Sabelström, F Huang, G Di Paolo, M Simons, JW Lewcock, C Haass
    EMBO Mol Med, 2020-03-10;0(0):e11227.
  4. TREM-1-accentuated lung injury via miR-155 is inhibited by LP17 nanomedicine
    Authors: Zhihong Yuan, Mansoor Syed, Dipti Panchal, Myungsoo Joo, Chetna Bedi, Sokbee Lim et al.
    American Journal of Physiology-Lung Cellular and Molecular Physiology
  5. The microglial innate immune receptors TREM-1 and TREM-2 in the anterior cingulate cortex (ACC) drive visceral hypersensitivity and depressive-like behaviors following DSS-induced colitis
    Authors: Wu, K;Liu, YY;Shao, S;Song, W;Chen, XH;Dong, YT;Zhang, YM;
    Brain, behavior, and immunity
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  6. TREM-1 amplifies corneal inflammation after Pseudomonas aeruginosa infection by modulating Toll-like receptor signaling and Th1/Th2-type immune responses.
    Authors: Wu M, Peng A, Sun M, Deng Q, Hazlett L, Yuan J, Liu X, Gao Q, Feng L, He J, Zhang P, Huang X
    Infect Immun, 2011-05-09;79(7):2709-16.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Bioassay, Western Blot
  7. TREM-1--expressing intestinal macrophages crucially amplify chronic inflammation in experimental colitis and inflammatory bowel diseases.
    Authors: Schenk M, Bouchon A, Seibold F, Mueller C
    J. Clin. Invest., 2007-10-01;117(10):3097-106.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  8. TREM-1 orchestrates angiotensin II–induced monocyte trafficking and promotes experimental abdominal aortic aneurysm
    Authors: Marie Vandestienne, Yujiao Zhang, Icia Santos-Zas, Rida Al-Rifai, Jeremie Joffre, Andreas Giraud et al.
    Journal of Clinical Investigation

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