Mouse TREML4/TLT-4 Antibody

Catalog #: MAB5500 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB5500
MAB5500-SP

Detection of TREML4 in Mouse Splenocytes by Flow Cytometry.

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All TREML4/TLT-4 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse TREML4/TLT-4 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse TREML4/TLT-4 in ELISAs.

Source

Monoclonal Rat IgG_2A Clone # 817914

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse TREML4/TLT-4
Gly25-Pro195
Accession # Q3LRV9

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

2.5 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of TREML4 in Mouse Splenocytes by Flow Cytometry. CD8⁺CD11c⁺mouse splenocytes were stained with Rat Anti-Mouse TREML4/TLT-4 Monoclonal Antibody (Catalog # MAB5500, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TREML4/TLT-4

TREML4 (Triggering Receptor Expressed on Myeloid cells-Like 4), also known as TLT4, is a 53-55 kDa member of the Ig superfamily. It is likely expressed by leukocytes, and based on its structure, may act as an activating receptor. Mature mouse TREML4 is a type I transmembrane (TM) glycoprotein that is 235 amino acids (aa) in length. It contains a 171 aa extracellular region (aa 29-199) with a V-type Ig-like domain (aa 29-122); the TM segment contains one Lys residue. There is one splice variant that shows a deletion of aa 21-24, a segment that lies in the putative signal sequence. Rodent and human TREML4 are highly divergent, and thus over aa 25-195, mouse TREML4 shares 78% aa sequence identity with rat TREML4, but only 52% aa sequence identity with human TREML4.

Long Name

Triggering Receptor Expressed on Myeloid Cells-like 4

Entrez Gene IDs

285852 (Human); 224840 (Mouse); 680862 (Rat)

Alternate Names

TLT4; TREML4