Mouse Tryptase-5/Prss32 Antibody

Catalog #: AF2634 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF2634
AF2634-SP

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Tryptase-5/Prss32 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Tryptase‑5/Prss32 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human Tryptase-G1, recombinant mouse (rm) MCPT-6, and rmMCPT‑7 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Tryptase‑5/Prss32
Ser20-Arg304
Accession # NP_081496

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse Tryptase‑5/Prss32 (Catalog # 2634-SE)

Immunoprecipitation

25 µg/mL

Conditioned cell culture medium spiked with Recombinant Mouse Tryptase‑5/Prss32 (Catalog # 2634-SE), see our available Western blot detection antibodies

Neutralization

Measured by its ability to neutralize Recombinant Mouse Tryptase‑5/Prss32 (2 µg/mL, Catalog # 2634-SE) cleavage of the fluorogenic peptide substrate Z-R-SBzl (100 µM). The Neutralization Dose (ND₅₀) is typically 24.3 µg/mL.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Tryptase-5/Prss32

Tryptase-5 is encoded by Prss32, one of 13 genes on mouse chromosome 17A3.3 that correspond to functional trypsin-like serine proteases (1). The deduced amino acid sequence of mouse Tryptase-5 consists of 331 residues with a signal peptide (residues 1 to 19), a pro region (residue 20 to 53), a catalytic domain (54 to 304), and a C-terminal hydrophobic peptide (residues 305 to 331). The mRNA is expressed in smooth muscle, eye, stomach, uterus and lymph node. Apparently, Prss32 does not seem to have a counterpart in the human genome.

References