Mouse TSLP Biotinylated Antibody

Catalog #: BAF555
7 Citations Datasheet / COA / SDS

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BAF555

All TSLP Antibodies

Product Details

Citations (7)

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse TSLP Biotinylated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse TSLP in ELISAs and Western blots. In sandwich immunoassays, less than 0.05% cross-reactivity with recombinant human (rh) TSLP, rhTSLP R, and recombinant mouse TSLP R is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse TSLP (R&D Systems, Catalog # 555-TSB)
Tyr20-Glu140
Accession # Q9JIE6

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse TSLP (Catalog # 555-TS)

Mouse TSLP Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Detection (Matched Antibody Pair)

0.1-0.4 µg/mL

Use in combination with:

Capture Reagent: Mouse TSLP Antibody (Catalog # MAB5551)

Standard: Recombinant Mouse TSLP Protein (Catalog # 555-TS)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TSLP

Thymic Stromal Lymphopoietin (TSLP) was originally identified as an activity from the conditioned medium of a mouse thymic stromal cell line that promoted the development of B cells. The activities of mouse TSLP overlap with, but are distinct from, those of mouse IL-7. Both mouse TSLP and IL-7 can co-stimulate growth of thymocytes and mature T cells, and support B lymphopoiesis in long-term cultures of fetal liver cells and bone-marrow cells. Whereas mouse IL-7 facilitates the development of B220+/IgM- pre-B cells, mouse TSLP promotes the development B220+/IgM+ B cells. Human TSLP was reported to preferentially stimulate myeloid cells; inducing the release of T cell-attracting chemokines from monocytes and enhancing the maturation of CD11c+ dendritic cells. Mouse TSLP cDNA encodes a 140 amino acid (aa) residue precursor protein with a 19 aa signal sequence. Within the mature region, there are three potential N-glycosylation sites and 7 cysteine residues. Mouse TSLP shares approximately 43% aa sequence identity with human TSLP. The gene for mouse TSLP has been localized to chromosome 18. By Northern and RT-PCR analysis, mouse TSLP expression has been detected in spleen, thymus, kidney, lung and bone marrow. TSLP is proposed to signal through a heterodimeric receptor complex that consists of IL-7 R alpha and the TSLP R, a new member of the hemopoietin receptor family most closely related to R gamma c.

References

Sims, J.E. et al. (2000) J. Exp. Med. 192:671.
Park, L.S. et al. (2000) J. Exp. Med. 192:659.
Pandey, A. et al. (2000) Nature Immunol. 1:59.
Reche, P.A. et al. (2001) J. Immunol. 167:336.

Long Name

Thymic Stromal Lymphopoietin

Entrez Gene IDs

85480 (Human); 53603 (Mouse); 102119254 (Cynomolgus Monkey)

Alternate Names

thymic stromal lymphopoietin; TSLP

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Mouse TSLP Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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IL-23 Induces Atopic Dermatitis-Like Inflammation Instead of Psoriasis-Like Inflammation in CCR2-Deficient Mice
Authors: Shannon K. Bromley, Ryan P. Larson, Steven F. Ziegler, Andrew D. Luster
PLoS ONE
Similarities and differences in the transcriptional control of expression of the mouse TSLP gene in skin epidermis and intestinal epithelium
Authors: KP Ganti, A Mukherji, M Surjit, M Li, P Chambon
Proc. Natl. Acad. Sci. U.S.A, 2017-01-23;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P
Response patterns of cytokines/chemokines in two murine strains after irradiation.
Authors: Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L
Cytokine, 2012-01-25;58(2):169-77.
Species: Mouse
Sample Types: Plasma
Applications: Luminex Development
Thymic stromal lymphopoietin is a key mediator of breast cancer progression.
Authors: Olkhanud PB, Rochman Y, Bodogai M
J. Immunol., 2011-04-13;186(10):5656-62.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P
Skin-derived TSLP triggers progression from epidermal-barrier defects to asthma.
Authors: Demehri S, Morimoto M, Holtzman MJ
PLoS Biol., 2009-05-19;7(5):e1000067.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P
Glyphosate-rich air samples induce IL-33, TSLP and generate IL-13 dependent airway inflammation
Authors: Sudhir Kumar, Marat Khodoun, Eric M. Kettleson, Christopher McKnight, Tiina Reponen, Sergey A. Grinshpun et al.
Toxicology
Thymic stromal lymphopoietin attenuates the development of atherosclerosis in ApoE-/- mice
Authors: Kunwu Yu, Pengfei Zhu, Qian Dong, Yucheng Zhong, Zhengfeng Zhu, Yingzhong Lin et al.
Journal of the American Heart Association