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Mouse TSLP Quantikine ELISA Kit

Catalog #: MTLP00
28 Citations 1 Review Datasheet / COA / SDS
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MTLP00
Mouse TSLP ELISA Standard Curve
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Product Details
Procedure
Citations (28)
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Reviews (1)
Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Mouse TSLP Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)
Sensitivity
6.3 pg/mL
Assay Range
7.8 - 500 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant mouse TSLP
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Mouse TSLP Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse TSLP in cell culture supernates, mouse serum, and plasma. It contains NS0-expressed recombinant mouse TSLP and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural mouse TSLP showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring mouse TSLP.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 31 31 31
Mean (pg/mL) 45 70 167 50 84 189
Standard Deviation 2.5 3.2 8.5 3.8 8.2 12.9
CV% 5.6 4.6 5.1 7.6 9.8 6.8

Recovery

The recovery of mouse TSLP spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=6) 103 93-114
EDTA Plasma (n=4) 94 80-108
Heparin Plasma (n=5) 96 89-119
Serum (n=5) 92 87-101

Linearity

To assess the linearity of the assay, samples spiked with high concentrations of mouse TSLP in each matrix were diluted with Calibrator Diluent and then assayed.
Mouse TSLP ELISA Linearity

Scientific Data

N/A Mouse TSLP ELISA Standard Curve View Larger

Mouse TSLP ELISA Standard Curve

Product Datasheets

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: TSLP

Thymic Stromal Lymphopoietin (TSLP) was originally identified as an activity from the conditioned medium of a mouse thymic stromal cell line that promoted the development of B cells. The activities of mouse TSLP overlap with, but are distinct from, those of mouse IL-7. Both mouse TSLP and IL-7 can co-stimulate growth of thymocytes and mature T cells, and support B lymphopoiesis in long-term cultures of fetal liver cells and bone-marrow cells. Whereas mouse IL-7 facilitates the development of B220+/IgM- pre-B cells, mouse TSLP promotes the development B220+/IgM+ B cells. Human TSLP was reported to preferentially stimulate myeloid cells, inducing the release of T cell-attracting chemokines from monocytes and enhancing the maturation of CD11c+ dendritic cells. Human TSLP cDNA encodes a 159 amino acid (aa) residue precursor protein with a 28 aa signal sequence. Within the mature region, six of the seven cysteine residues present in the mouse TSLP involved in intramolecular disulfide bond formation are conserved in the human TSLP. Human TSLP shares approximately 43% aa sequence identity with mouse TSLP. By Northern blot analysis, human TSLP expression has been detected in many tissues with the highest expressions in heart, liver, testis and prostate. TSLP signals through a heterodimeric receptor complex that consists of IL-7 R alpha and the TSLP R, a member of the hemopoietin receptor family most closely related to Rgammac.

TSLP R, also named Delta and CRLM-2 (Cytokine Receptor-like Module-2), was originally cloned as a novel type 1 cytokine receptor with similarity to the common gamma chain (gamma c). It was subsequently identified as a subunit of the cellular receptor for the IL-7-like cytokine TSLP and termed TSLP R. TSLP R shows 24% sequence identity with the mouse gc receptor. TSLP R expression is constitutive in immune and hematopoietic cells, but is up-regulated in Th2 cells.

Long Name:
Thymic Stromal Lymphopoietin
Entrez Gene IDs:
85480 (Human); 53603 (Mouse); 102119254 (Cynomolgus Monkey)
Alternate Names:
thymic stromal lymphopoietin; TSLP
&#9888; WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 50 µL Assay Diluent
  3.   Add 50 µL of Assay Diluent to each well.
    4. 50 µL Standard, Control, or Sample
  4.   Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  5.   Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.
    6. 100 µL Conjugate
  6.   Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate and wash 5 times.
    8. 100 µL Substrate Solution
  8.   Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
    9. 100 µL Stop Solution
  9.   Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Mouse TSLP Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

28 Citations: Showing 1 - 10
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  1. The microbiota and T cells non-genetically modulate inherited phenotypes transgenerationally
    Authors: Harris, JC;Trigg, NA;Goshu, B;Yokoyama, Y;Dohnalová, L;White, EK;Harman, A;Murga-Garrido, SM;Ting-Chun Pan, J;Bhanap, P;Thaiss, CA;Grice, EA;Conine, CC;Kambayashi, T;
    Cell reports
    Species: Mouse, Transgenic Mouse
    Sample Types: Serum
  2. Fallopia japonica Root Extract Ameliorates Ovalbumin-Induced Airway Inflammation in a CARAS Mouse Model by Modulating the IL-33/TSLP/NF-?B Signaling Pathway
    Authors: Jin, J;Fan, YJ;Nguyen, TV;Yu, ZN;Song, CH;Lee, SY;Shin, HS;Chai, OH;
    International journal of molecular sciences
    Species: Mouse
    Sample Types: BALF, NLF
  3. Increased hepatic interleukin-1, arachidonic acid, and reactive oxygen species mediate the protective potential of peptides shared by gut cysteine peptidases against Schistosoma mansoni infection in mice
    Authors: H Tallima, R El Ridi
    PloS Neglected Tropical Diseases, 2023-03-15;17(3):e0011164.
    Species: Mouse
    Sample Types: Tissue Homogenates
  4. Crosstalk between ILC2s and Th2 cells varies among mouse models
    Authors: RK Gurram, D Wei, Q Yu, MJ Butcher, X Chen, K Cui, G Hu, M Zheng, X Zhu, J Oh, B Sun, JF Urban, K Zhao, WJ Leonard, J Zhu
    Cell Reports, 2023-02-02;42(2):112073.
    Species: Mouse
    Sample Types: BALF
  5. Downregulation of deubiquitinating enzyme USP25 promotes the development of allergic rhinitis by enhancing TSLP signaling in the nasal epithelium
    Authors: W Chang, H Lv, L Tan, Z Gao, P Liu, D Qin, W Zhang, Y Xu
    Molecular Medicine Reports, 2022-09-30;26(5):.
    Species: Mouse, Transgenic Mouse
    Sample Types: Cell Culture Supernates
  6. Roseburia intestinalis stimulates TLR5-dependent intestinal immunity against Crohn's disease
    Authors: Z Shen, W Luo, B Tan, K Nie, M Deng, S Wu, M Xiao, X Wu, X Meng, T Tong, C Zhang, K Ma, Y Liao, J Xu, X Wang
    EBioMedicine, 2022-09-28;85(0):104285.
    Species: Mouse
    Sample Types: Serum
  7. Effects of the dipeptide L-glutamic acid-L-tryptophan on dermatitis in mice and human keratinocytes
    Authors: S Shibata, A Kuwahara, M Sakaki-Yum, M Kawaguchi, T Ishii, Y Honma
    Heliyon, 2022-01-07;8(1):e08729.
    Species: Mouse
    Sample Types: Serum
  8. Eosinophil extracellular traps drive asthma progression through neuro-immune signals
    Authors: Y Lu, Y Huang, J Li, J Huang, L Zhang, J Feng, J Li, Q Xia, Q Zhao, L Huang, S Jiang, S Su
    Nature Cell Biology, 2021-10-06;23(10):1060-1072.
    Species: Mouse
    Sample Types: BALF
  9. Neutralization of IL-33 modifies the type 2 and type 3 inflammatory signature of viral induced asthma exacerbation
    Authors: KJ Warren, JA Poole, JM Sweeter, JM DeVasure, JD Dickinson, RS Peebles, TA Wyatt
    Respiratory Research, 2021-07-15;22(1):206.
    Species: Mouse
    Sample Types: BALF
  10. COX Inhibition Increases Alternaria-Induced Pulmonary Group 2 Innate Lymphoid Cell Responses and IL-33 Release in Mice
    Authors: W Zhou, J Zhang, S Toki, K Goleniewsk, AE Norlander, DC Newcomb, P Wu, KL Boyd, H Kita, RS Peebles
    J. Immunol., 2020-07-20;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  11. TSLP protects against liver I/R injury via activation of the PI3K/Akt pathway
    Authors: S Li, Z Yi, M Deng, MJ Scott, C Yang, W Li, Z Lei, NM Santerre, P Loughran, TR Billiar
    JCI Insight, 2019-11-14;4(22):.
    Species: Mouse
    Sample Types: Serum
  12. Role of bulge epidermal stem cells and TSLP signaling in psoriasis
    Authors: N Gago-Lopez, LF Mellor, D Megías, G Martín-Ser, A Izeta, F Jimenez, EF Wagner
    EMBO Mol Med, 2019-09-26;0(0):e10697.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  13. TSLP Protects Corneas From Pseudomonas aeruginosa Infection by Regulating Dendritic Cells and IL-23-IL-17 Pathway
    Authors: X Cui, N Gao, R Me, J Xu, FX Yu
    Invest. Ophthalmol. Vis. Sci., 2018-08-01;59(10):4228-4237.
    Species: Mouse
    Sample Types:
  14. STAT1 Represses Cytokine-Producing Group 2 and Group 3 Innate Lymphoid Cells during Viral Infection
    Authors: MT Stier, K Goleniewsk, JY Cephus, DC Newcomb, TP Sherrill, KL Boyd, MH Bloodworth, ML Moore, K Chen, JK Kolls, RS Peebles
    J. Immunol., 2017-06-02;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  15. Similarities and differences in the transcriptional control of expression of the mouse TSLP gene in skin epidermis and intestinal epithelium
    Authors: KP Ganti, A Mukherji, M Surjit, M Li, P Chambon
    Proc. Natl. Acad. Sci. U.S.A, 2017-01-23;0(0):.
    Species: Mouse
    Sample Types: Serum
  16. Innate sensing of microbial products promotes wound-induced skin cancer.
    Authors: Hoste E, Arwert E, Lal R, South A, Salas-Alanis J, Murrell D, Donati G, Watt F
    Nat Commun, 2015-01-09;6(0):5932.
    Species: Mouse
    Sample Types: Serum
  17. Epidermal barrier defects link atopic dermatitis with altered skin cancer susceptibility.
    Authors: Cipolat S, Hoste E, Natsuga K, Quist S, Watt F
    Elife, 2014-05-05;3(0):e01888.
    Species: Mouse
    Sample Types: Serum
  18. Airway uric acid is a sensor of inhaled protease allergens and initiates type 2 immune responses in respiratory mucosa.
    Authors: Hara K, Iijima K, Elias M, Seno S, Tojima I, Kobayashi T, Kephart G, Kurabayashi M, Kita H
    J Immunol, 2014-03-24;192(9):4032-42.
    Species: Mouse
    Sample Types: Tissue Homogenates
  19. Loss of Grainy head-like 1 is associated with disruption of the epidermal barrier and squamous cell carcinoma of the skin.
    Authors: Mlacki, Michal, Darido, Charbel, Jane, Stephen, Wilanowski, Tomasz
    PLoS ONE, 2014-02-20;9(2):e89247.
    Species: Mouse
    Sample Types: Whole Cells
  20. IL-33-responsive lineage- CD25+ CD44(hi) lymphoid cells mediate innate type 2 immunity and allergic inflammation in the lungs.
    Authors: Bartemes KR, Iijima K, Kobayashi T, Kephart GM, McKenzie AN, Kita H
    J. Immunol., 2011-12-23;188(3):1503-13.
    Species: Mouse
    Sample Types: BALF
  21. IL-22 Is Produced by Innate Lymphoid Cells and Limits Inflammation in Allergic Airway Disease.
    Authors: Taube C, Tertilt C, Gyulveszi G, Dehzad N, Kreymborg K, Schneeweiss K, Michel E, Reuter S, Renauld JC, Arnold-Schild D, Schild H, Buhl R, Becher B
    PLoS ONE, 2011-07-18;6(7):e21799.
    Species: Mouse
    Sample Types: BALF
  22. Thymic stromal lymphopoietin is produced by dendritic cells.
    Authors: Kashyap M, Rochman Y, Spolski R
    J. Immunol., 2011-06-20;187(3):1207-11.
    Species: Mouse
    Sample Types: BALF
  23. Thymic stromal lymphopoietin contributes to myeloid hyperplasia and increased immunoglobulins, but not epidermal hyperplasia, in RabGEF1-deficient mice.
    Authors: Tsai M, Chen CC, Mukai K
    Am. J. Pathol., 2010-09-09;177(5):2411-20.
    Species: Mouse
    Sample Types: Tissue Homogenates
  24. Thymic stromal lymphopoietin is a critical mediator of IL-13-driven allergic inflammation.
    Authors: Miyata M, Nakamura Y, Shimokawa N, Ohnuma Y, Katoh R, Matsuoka S, Okumura K, Ogawa H, Masuyama K, Nakao A
    Eur. J. Immunol., 2009-11-01;39(11):3078-83.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  25. Epidermal Notch1 loss promotes skin tumorigenesis by impacting the stromal microenvironment.
    Authors: Demehri S, Turkoz A, Kopan R
    Cancer Cell, 2009-07-07;16(1):55-66.
    Species: Mouse
    Sample Types: Serum
  26. Skin-derived TSLP triggers progression from epidermal-barrier defects to asthma.
    Authors: Demehri S, Morimoto M, Holtzman MJ
    PLoS Biol., 2009-05-19;7(5):e1000067.
    Species: Mouse
    Sample Types: Serum
  27. A small molecule CRTH2 antagonist inhibits FITC-induced allergic cutaneous inflammation.
    Authors: Boehme SA, Franz-Bacon K, Chen EP, Sasik R, Sprague LJ, Ly TW, Hardiman G, Bacon KB
    Int. Immunol., 2008-12-09;21(1):81-93.
    Species: Mouse
    Sample Types: Tissue Homogenates
  28. A potential role of thymic stromal lymphopoietin in the recruitment of macrophages to mouse intervertebral disc cells via monocyte chemotactic protein 1 induction: implications for herniated discs.
    Authors: Ohba T, Haro H, Ando T, Koyama K, Hatsushika K, Suenaga F, Ohnuma Y, Nakamura Y, Katoh R, Ogawa H, Hamada Y, Nakao A
    Arthritis Rheum., 2008-11-01;58(11):3510-9.
    Species: Mouse
    Sample Types: Cell Culture Supernates

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Mouse TSLP Quantikine ELISA Kit
By Josh Richards on 02/26/2020
Sample Tested: Cell culture supernatant

Used Mouse TSLP ELISA for analysing organoid supernatants.
Very easy to use kit, straight forward instructions and nice results. Standard curve came out very nicely. Would highly recommend.

Mouse TSLP Quantikine ELISA Kit MTLP00