Mouse Uromodulin Antibody

Detection of Mouse Uromodulin by Western Blot. Western blot shows lysates of mouse kidney tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse Uromodulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5175) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). A specific band was detected for Uromodulin at approximately 115 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Uromodulin in Mouse Kidney. Uromodulin was detected in immersion fixed frozen sections of mouse kidney using Sheep Anti-Mouse Uromodulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5175) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; NL010) and counterstained with DAPI (blue). Specific staining was localized to convoluted tubule epithelial cells. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Detection of Mouse Uromodulin by Simple Western^TM. Simple Western shows lysates of mouse kidney, loaded at 0.5 mg/ml. A specific band was detected for Uromodulin at approximately 182 kDa (as indicated) using 20 µg/mL of Sheep Anti-Mouse Uromodulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5175). This experiment was conducted under reducing conditions and using the 12-230kDa separation system.

Detection of Mouse Uromodulin by Immunocytochemistry/Immunofluorescence Intraparenchymal delivery of lentiviral vectors preferentially infects epithelial cells of both kidneys and urinary bladder.(a) Confocal images of Strawberry expression in renal sections of ELS infected kidneys 60 days post injection. Sections were stained with the indicated antibodies and merged images with DAPI (blue) are presented. Preferential transduction was observed in proximal (AQP1) and distal (THP) renal tubular epithelial cells and renal fibroblasts (CD73) (white arrowheads). Strawberry was not localised to collecting ducts (AQP2) or podocytes (Nephrin) (white arrows). Scale bars, 50 μm. (b) Confocal images of Strawberry expression in the liver, spleen, lung, urinary bladder and left and right kidney of ELS infected animals 60 days post left intrarenal injection (top panels) and uninjected control animals (bottom panels). Sections were stained with an antibody against Strawberry (green) and merged images with DAPI (blue) are presented. Scale bars, 50 μm. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/26046460), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Mouse Uromodulin by Immunohistochemistry Pax8-CreERT2 mediated beta -galactosidase expression.(A) Enzymatic X-gal staining of cryosections of kidneys (top panels) derived from 10 week old Pax8-CreERT2/Rosa26R mice either induced with tamoxifen (a, renal parenchyma; b, collecting ducts) or uninduced (c). Tamoxifen administration did not induce beta -galactosidase expression in any of the other major organs examined (representative tissues presented in bottom panels). (B) beta -galactosidase positivity was exclusively found in the tubular epithelium of the kidney of tamoxifen treated mice, with no staining observed in glomeruli or bloods vessels (a, framed area is shown enlarged in b). Kidneys from vehicle treated mice were negative (c). Co-localisation studies revealed X-gal expression within tubules of all renal tubular compartment (proximal tubules—AQP1, distal tubules–THP and collecting ducts–AQP2). Scale bars, 100μm. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/26866916), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Mouse Uromodulin by Immunohistochemistry Slc22a6-CreERT2 mediated beta -galactosidase expression.(A) Enzymatic X-gal staining of cryosections of kidneys (top panels) derived from 10 week old Slc22a6-CreERT2/Rosa26R mice either induced with tamoxifen (a, renal cortex; b, medulla) or uninduced (c, renal cortex). Tamoxifen administration did not induce beta -galactosidase expression in any of the other major organs examined (representative tissues presented in bottom panels). (B) beta -galactosidase positivity was exclusively found in the tubular epithelium of the kidney of tamoxifen treated mice (a); kidneys from vehicle treated mice were negative (b). Co-localisation studies revealed specific X-gal expression within proximal tubular epithelium (AQP1) with no expression detected in distal tubules (THP) or collecting ducts (AQP2). Scale bars, 100μm. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/26866916), licensed under a CC-BY license. Not internally tested by R&D Systems.