Mouse VG5Q Antibody

Catalog #: MAB30481 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB30481
MAB30481-SP

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Mouse VG5Q Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse VG5Q in direct ELISAs.

Source

Monoclonal Rat IgG_2A Clone # 368011

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant mouse VG5Q
Met1-Glu711
Accession # Q7TN31

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunocytochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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VG5Q in bEnd.3 Mouse Cell Line. VG5Q was detected in immersion fixed bEnd.3 mouse endothelioma cell line using Rat Anti-Mouse VG5Q Monoclonal Antibody (Catalog # MAB30481) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VG5Q

Angiogenic Factor VG5Q, also known as AGGF1, is a presumably secreted protein expressed by vascular endothelial cells. Mouse VG5Q cDNA encodes a 714 aa precursor with a coiled coil domain, a forkhead (FHA) domain, and RNA-association domains (G-patch, OCRE). VG5Q binds the angiogenic TNF superfamily ligand TWEAK, and promotes endothelial cell proliferation. Human VG5Q mutations are associated with Klippel-Trenaunay syndrome, a congenital vascular morphogenesis disorder. Mouse VG5Q shares 78% aa identity with human VG5Q.

Long Name

Vasculogenesis Gene on 5q/Angiogenic Factor with G Patch and FHA Domains 1

Entrez Gene IDs

55109 (Human)

Alternate Names

AGGF1; Angiogenic factor VG5Q; angiogenic factor with G patch and FHA domains 1; FLJ10283hVG5Q; GPATC7G patch domain-containing protein 7; GPATCH7HUS84971; HSU84971; VG5Q; VG5QVasculogenesis gene on 5q protein