Mouse VISTA/B7-H5/PD-1H Antibody

Catalog #: MAB70051 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB70051
MAB70051-SP

Detection of Mouse VISTA/B7-H5/PD-1H by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse VISTA/B7-H5/PD-1H Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse VISTA/B7-H5/PD-1H in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant human VISTA/B7-H5/PD-1H is observed.

Source

Monoclonal Rat IgG_2A Clone # 742002

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse VISTA/B7-H5/PD-1H
Phe33-Ala191
Accession # Q9D659

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.5 µg/mL

See below

Immunocytochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Mouse VISTA/B7-H5/PD-1H by Western Blot. Western blot shows lysates of mouse placenta tissue, mouse spleen tissue, mouse lung tissue, and mouse platelets. PVDF membrane was probed with 0.5 µg/mL of Rat Anti-Mouse VISTA/B7-H5/ PD-1H Monoclonal Antibody (Catalog # MAB70051) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for VISTA/B7-H5/PD-1H at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry

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VISTA/B7-H5/PD-1H in D3 Mouse Stem Cells. VISTA/B7-H5/PD-1H was detected in immersion fixed D3 mouse embryonic stem cell line using Rat Anti-Mouse VISTA/B7-H5/PD-1H Monoclonal Antibody (Catalog # MAB70051) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and cell surfaces. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VISTA/B7-H5/PD-1H

Platelet Receptor Gi24, also known as VISTA, B7-H5, B7H5, Dies1 (Differentiation of ESC-1), SISP1 and C10orf54, is a 55-65 kDa member of the Ig superfamily. It is a transmembrane molecule expressed in bone, on embryonic stem cells (ESCs), and on tumor cell surfaces. On ESCs, Gi24 appears to positively interact with BMP4, potentiating BMP signaling and the transition from an undifferentiated to a differentiated state. On tumor cells, Gi24 both promotes MT1-MMP expression and activity, and serves as a substrate for MT1-MMP. This increases the potential for cell motility. Mature mouse Gi24 is a 277 amino acid (aa) type I transmembrane glycoprotein (aa 33-309). It contains a 149 aa extracellular region (aa 33-191) with one V-type Ig-like domain (aa 33-161) and a 97 aa cytoplasmic domain. Based on human Gi24, mouse Gi24 will likely undergo proteolytic cleavage by MT1-MMP, generating a soluble 30 kDa extracellular fragment, plus a 25-30 kDa membrane-bound fragment. There are two potential isoform variants. One contains a deletion of aa 127-187, while another shows an alternative start site at Met82. Over aa 33-191, mouse Gi24/Dies1 shares 78% and 70% aa identity with rat and human Gi24, respectively.

Entrez Gene IDs

64115 (Human); 74048 (Mouse); 690899 (Rat); 102116468 (Cynomolgus Monkey)

Alternate Names

4632428N05Rik; B7H5; B7-H5; C10orf54; chromosome 10 open reading frame 54; Dies1; Gi24; PD1H; PD-1H; platelet receptor Gi24; PP2135; SISP1; stress induced secreted protein 1; VISTA; VSIR