Mouse Wnt-10a Antibody

Name: Mouse Wnt-10a Antibody
Brand: R&D Systems
SKU: MAB3469

Catalog #: MAB3469
1 Citation Datasheet / COA / SDS

Discontinued Product

MAB3469 has been discontinued.

View all Wnt-10a products.

Detection of Mouse Wnt‑10a by Western Blot.

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All Wnt-10a Antibodies

Product Details

Citations (1)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Wnt-10a Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Wnt-10a in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human Wnt-2, -7a, -7b, recombinant mouse Wnt-1, -3a, -4, -5a, -5b, -8a, -8b, or -9a is observed. Western blotting with human cell extracts demonstrates that this antibody also detects human Wnt-10a.

Source

Monoclonal Rat IgG_2A Clone # 342825

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant mouse Wnt‑10a
Leu55-Glu95 and Gln278-Arg360
Accession # P70701

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Applications

Recommended Concentration

Sample

Western Blot

5 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Mouse Wnt‑10a by Western Blot. Western blot shows lysates of Raji human Burkitt's lymphoma cell line and SW480 human bladder cancer cell line. PVDF membrane was probed with 5 µg/mL Rat Anti-Mouse Wnt-10a Monoclonal Antibody (Catalog # MAB3469) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). For additional reference, recombinant mouse Wnt-10a (2 ng) was included. A specific band for Wnt-10a was detected at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 6.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Wnt-10a

Wnt-10a is a presumably secreted, glycosylated member of the Wnt family of developmental proteins. Mouse Wnt-10a is synthesized as a 417 amino acid (aa) precursor that contains a 382 aa mature region. The mature region contains 24 cysteines and two potential N-linked glycosylation sites. Mature mouse Wnt-10a shares 96% and 99% aa sequence identity with mature human and rat Wnt-10a, respectively. Wnt-10a shares 64% aa sequence identity with Wnt-10b/12. Wnt-10a is produced by tumors and thymic epithelium.

Long Name

Wingless-type MMTV Integration Site Family Member 10A

Entrez Gene IDs

80326 (Human); 22409 (Mouse); 316527 (Rat)

Alternate Names

FLJ14301; protein Wnt-10a; SSPS; wingless-type MMTV integration site family, member 10A; Wnt10a; Wnt-10a

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Mouse Wnt-10a Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Caveolin-1 (P132L), a common breast cancer mutation, confers mammary cell invasiveness and defines a novel stem cell/metastasis-associated gene signature.
Authors: Bonuccelli G, Casimiro MC, Sotgia F, Wang C, Liu M, Katiyar S, Zhou J, Dew E, Capozza F, Daumer KM, Minetti C, Milliman JN, Alpy F, Rio MC, Tomasetto C, Mercier I, Flomenberg N, Frank PG, Pestell RG, Lisanti MP
Am. J. Pathol., 2009-05-01;174(5):1650-62.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot