Mouse Wnt-10b Antibody

Catalog #: MAB2110
3 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB2110
MAB2110-SP

All Wnt-10b Antibodies

Product Details

Citations (3)

Supplemental Products

Reviews

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Wnt-10b Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Wnt-10b in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse Wnt-1, -3a, -4,
-5a, -5b, -8a, -11, or -16 is observed.

Source

Monoclonal Rat IgG_2B Clone # 254206

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant mouse Wnt-10b
Arg263-Arg332
Accession # P48614

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Mouse Wnt‑10b

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Wnt-10b

Wnt-10b, also known as Wnt-12, is a 45 kDa glycoprotein involved in fetal limb bud development and adult hematopoiesis. Mouse Wnt-10b exists in two isoforms that are distinguished by the presence or absence of an internal 95 amino acid sequence.

Long Name

Wingless-type MMTV Integration Site Family, Member 10B

Entrez Gene IDs

7480 (Human); 22410 (Mouse)

Alternate Names

protein Wnt-10b; Protein Wnt-12; SHFM6WNT-10B protein; wingless-type MMTV integration site family, member 10B; Wnt10b; Wnt-10b; WNT12; Wnt-12

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Mouse Wnt-10b Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Wnt10b activates the wnt, notch, and NF kappa B pathways in u2os osteosarcoma cells
Authors: Ulrike I. Mödder, Merry Jo Oursler, Sundeep Khosla, David G. Monroe
Journal of Cellular Biochemistry
Regulation of cyclin D1 and Wnt10b gene expression by cAMP-responsive element-binding protein during early adipogenesis involves differential promoter methylation.
Authors: Fox KE, Colton LA, Erickson PF, Friedman JE, Cha HC, Keller P, MacDougald OA, Klemm DJ
J. Biol. Chem., 2008-10-27;283(50):35096-105.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot
Msx2 exerts bone anabolism via canonical Wnt signaling.
Authors: Cheng SL, Shao JS, Cai J, Sierra OL, Towler DA
J. Biol. Chem., 2008-05-15;283(29):20505-22.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot