Key Product Details
Species Reactivity
Mouse
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 254126
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Product Specifications
Immunogen
E. coli-derived recombinant mouse Wnt-8a
Ala20-Trp354 (predicted)
Accession # Q64527
Ala20-Trp354 (predicted)
Accession # Q64527
Specificity
Detects mouse Wnt-8a in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant mouse Wnt-1, -3a, -4, -5a, or -10b is observed.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Applications for Mouse Wnt‑8a Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Recombinant Mouse Wnt‑8a
Sample: Recombinant Mouse Wnt‑8a
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: Wnt-8a
Long Name
Wingless-type MMTV Integration Site Family, Member 8a
Alternate Names
Wnt8a
Gene Symbol
WNT8A
UniProt
Additional Wnt-8a Products
Product Documents for Mouse Wnt‑8a Antibody
Product Specific Notices for Mouse Wnt‑8a Antibody
For research use only
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars