Mouse XCL1/Lymphotactin Antibody

Catalog #: MAB486
3 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB486
MAB486-SP

Product Details

Citations (3)

Supplemental Products

Reviews

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse XCL1/Lymphotactin Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse XCL1/Lymphotactin in ELISAs and Western blots. In sandwich immunoassays, less than 1% cross-reactivity with recombinant human XCL1/Lymphotactin is observed and no cross-reactivity with recombinant viral (rv) CMV UL147 is observed. In Western blots, 75% cross‑reactivity with rvCMV UL147 is observed and no cross-reactivity with recombinant human CCL1-3, 5, 7, 8, 11, 13-25, 28, recombinant mouse CCL1-7, 9, 11, 12, 15, 19, 21, 22, 24, 25, 27 or 28 is observed.

Source

Monoclonal Rat IgG_2A Clone # 80222

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant mouse XCL1/Lymphotactin
Val22-Gly114
Accession # AAA56752

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Mouse XCL1/Lymphotactin (Catalog # 486-LT)

Mouse XCL1/Lymphotactin Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Capture (Matched Antibody Pair)

2-8 µg/mL

Use in combination with:

Detection Reagent: Mouse XCL1/Lymphotactin Biotinylated Antibody (Catalog # BAF486)

Standard: Recombinant Mouse XCL1/Lymphotactin Protein (Catalog # 486-LT)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: XCL1/Lymphotactin

Mouse XCL1, also known as Lymphotactin, and its human homologue (also named human SCM-1 and ATAC) belong to the C or gamma subfamily of chemokines. The C chemokines lack two (the 1st and 3rd) of the four invariant cysteine residues normally found in the CC and CXC chemokines and have an extended carboxy terminus. Mouse XCL1 encodes a 114 amino acid residue precursor protein with a 21 amino acid residue predicted signal peptide. The expression of XCL1 is restricted to activated mouse pro-T cells and to activated, class I MHC restricted T cells. The gene for XCL1 has been mapped to chromosome 1 in both human and mouse. Recombinant mouse XCL1 has been shown to have chemotactic activity for lymphocytes, NK cells and mouse splenocytes.

References

Kelner, G. et al. (1994) Science 266:1395.
Hedrick, J.A. et al. (1997) J. Immunol. 158:1533.

Long Name

Chemokine [C Motif] Ligand 1

Entrez Gene IDs

6375 (Human); 16963 (Mouse)

Alternate Names

ATAC; ATACSmall-inducible cytokine C1; C motif chemokine 1; chemokine (C motif) ligand 1; Cytokine SCM-1; LPTNSCM-1-alpha; LTNXC chemokine ligand 1; Lymphotactin; lymphotaxin; SCM-1 alpha; SCM-1; SCM1A; SCM-1a; SCYC1SCM1; single cysteine motif 1a; small inducible cytokine subfamily C, member 1 (lymphotactin); XCL1

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Mouse XCL1/Lymphotactin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
Filter your results:

Filter by:

XCR1+ DCs are critical for Tï¿½cell-mediated immunotherapy of chronic viral infections
Authors: E Domenjo-Vi, V Casella, R Iwabuchi, E Fossum, M Pedragosa, Q Castellví, P Cebollada, T Kaisho, K Terahara, G Bocharov, J Argilaguet, A Meyerhans
Oncogene, 2023-02-14;42(2):112123.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
XCR1+ type 1 conventional dendritic cells drive liver pathology in non-alcoholic steatohepatitis
Authors: A Deczkowska, E David, P Ramadori, D Pfister, M Safran, B At The, A Giladi, DA Jaitin, O Barboy, M Cohen, I Yofe, C Gur, S Shlomi-Lou, S Henri, Y Suhail, M Qiu, S Kam, H Hermon, E Lahat, G Ben Yakov, O Cohen-Ezra, Y Davidov, M Likhter, D Goitein, S Roth, A Weber, B Malissen, A Weiner, Z Ben-Ari, M Heikenwäld, E Elinav, I Amit
Nature Medicine, 2021-05-20;0(0):.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization
NK Cells Stimulate Recruitment of cDC1 into the Tumor Microenvironment Promoting Cancer Immune Control
Authors: JP Böttcher, E Bonavita, P Chakravart, H Blees, M Cabeza-Cab, S Sammicheli, NC Rogers, E Sahai, S Zelenay, C Reis E Sou
Cell, 2018-02-08;172(5):1022-1037.e14.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization