NADA-green
Chemical Name: 3-[(7-Nitro-2,1,3-benzoxadiazol-4-yl)amino]-D-alanine hydrochloride
Purity: ≥95%
Biological Activity
NADA-green is a fluorescent D-amino acid. Suitable for labeling peptidoglycans in live bacteria. Incorporated into bacterial cell walls during synthesis. Results in strong peripheral and septal labeling of taxonomically diverse bacterial cell populations without affecting growth rate. Excitation/emission λ ~450/555 nm.Please note that the spectral data is solvent dependant
Optical Data for NADA-green
| Emission Color | Green |
|---|---|
| λabs | 450 nm |
| λem | 555 nm |
| Extinction Coefficient (ε) | 25,000 M-1cm-1 |
| Application | Fluorescence Microscopy |
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
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Technical Data
The technical data provided above is for guidance only.
For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Background References
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In situ probing of newly synthesized peptidoglycan in live bacteria with fluorescent D-amino acids.
Kuru et al.
Angew.Chem.Int.Ed., 2012;51:12519 -
Synthesis of fluorescent D-amino acids and their use for probing peptidoglycan synthesis and bacterial growth in situ.
Kuru et al.
Nat.Protoc., 2014;10:33 -
Determining the Membrane Topology of Proteins: Insertion Pathway of a Transmembrane Helix of Annexin 12
Ladokhin et al.
Biochemistry, 2002;41:13617
Product Datasheets
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Citations for NADA-green
The citations listed below are publications that use Tocris products. Selected citations for NADA-green include:
2 Citations: Showing 1 - 2
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Maturing Mycobacterium smegmatis peptidoglycan requires non-canonical crosslinks to maintain shape.
Authors: Baranowski Et al.
ELife 2018;7
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Peptidoglycan precursor synthesis along the sidewall of pole-growing mycobacteria.
Authors: Garcia-Heredia Et al.
ELife 2018;7
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