NanoLuc® (Nluc) Luciferase Antibody Summary
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of NanoLuc® Luciferase by Western Blot. Western blot shows Recombinant NanoLuc®. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human NanoLuc® (Nluc) Luciferase Monoclonal Antibody (Catalog # MAB100261) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for NanoLuc® at approximately 20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Luciferase
NanoLuc® (Nluc) luciferase is a small enzyme (19.1kDa) engineered for optimal performance as a luminescent reporter. NanoLuc® Binary Technology (NanoBiT™) is a two subunit system based on NanoLuc® luciferase. Large BiT (LgBiT; 17.6kDa) is the large subunit of the NanoBiT® system.
Product Datasheets
Product Specific Notices
NanoLuc and NanoBiT are registered trademarks of Promega Corporation.Citations for NanoLuc® (Nluc) Luciferase Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 2
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NanoLuc Binary Technology as a methodological approach: an important new tool for studying the localization of androgen receptor and androgen receptor splice variant V7 homo and heterodimers
Authors: Guzman, J;Weigelt, K;Neumann, A;Tripal, P;Schmid, B;Winter, Z;Palmisano, R;Culig, Z;Cronauer, MV;Muschler, P;Wullich, B;Taubert, H;Wach, S;
BMC cancer
Species: Human
Sample Types: Whole Cells
Applications: Immunocytochemistry -
Frizzled BRET sensors based on bioorthogonal labeling of unnatural amino acids reveal WNT-induced dynamics of the cysteine-rich domain
Authors: M Kowalski-J, H Schihada, A Turku, T Huber, TP Sakmar, G Schulte
Science Advances, 2021-11-10;7(46):eabj7917.
Species: Human
Sample Types: Cell Lysates, Whole Cells
Applications: ELISA Capture, Western Blot
FAQs
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What is the immunogen for the NanoLuc® (Nluc) Luciferase Antibody, Catalog # MAB100261? Does this antibody recognize LgBit or SmBit?
The immunoogen for MAB100261 is a Synthetic peptide of NanoLuc® (Nluc) Luciferase. For more details on the compound, Promega Corporation should be contacted. NanoBit (LgBit+SmBit) is a derivative of the original product NanoLuc, where portion of the protein is split to get two complementary peptides. MAB100261 detects an epitope on NanoLuc that is not part of LgBit or SmBit. SmBit is only 11 amino acids in length.
Reviews for NanoLuc® (Nluc) Luciferase Antibody
Average Rating: 5 (Based on 1 Review)
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Really nice antibody to work with. It gives you really bright pictures.
