Normal Chicken IgY Control

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AB-101-C
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R&D Systems Antibodies
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Product Details
Citations (23)
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Normal Chicken IgY Control Summary

Specificity
Egg yolks were obtained from naive (non-immunized) chickens and purified for use as normal chicken IgY.
Source
Polyclonal Chicken IgY
Purification
Ion exchange-purified from egg yolks
Immunogen
NA
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Control
Negative control for use in conjunction with R&D Systems antibodies. Use at the same concentration as the detection antibody.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Dissolve the lyophilized chicken IgY in sterile PBS, pH 7.4. If 1 mL of PBS is used, the antibody concentration will be 1 mg/mL.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IgY

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin Y
Alternate Names
IgY

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Citations for Normal Chicken IgY Control

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

23 Citations: Showing 1 - 10
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  1. Boosting peripheral BDNF rescues impaired in vivo axonal transport in CMT2D mice
    Authors: JN Sleigh, D Villarroel, S Surana, T Wickenden, Y Tong, RL Simkin, JNS Vargas, ER Rhymes, AP Tosolini, SJ West, Q Zhang, XL Yang, G Schiavo
    JCI Insight, 2023-05-08;0(0):.
  2. Senescence-related deterioration of intercellular junctions in the peritoneal mesothelium promotes the transmesothelial invasion of ovarian cancer cells
    Authors: M Paku?a, A Witucka, P Uruski, A Radziemski, R Moszy?ski, D Szpurek, K Maksin, A Wo?niak, S Sajdak, A Tykarski, J Miku?a-Pie, K Ksi??ek
    Sci Rep, 2019-05-20;9(1):7587.
  3. Slow Interstitial Fluid Flow Activates TGF-? Signaling and Drives Fibrotic Responses in Human Tenon Fibroblasts
    Authors: Wiedenmann, CJ;Gottwald, C;Zeqiri, K;Frömmichen, J;Bungert, E;Gläser, M;Ströble, J;Lohmüller, R;Reinhard, T;Lübke, J;Schlunck, G;
    Cells
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization Control
  4. A Lateral Flow Assay for the Detection of Leptospira lipL32 Gene Using CRISPR Technology
    Authors: Satheesh Natarajan, Jayaraj Joseph, Balamurugan Vinayagamurthy, Pedro Estrela
    Sensors (Basel)
  5. Bone marrow and periosteal skeletal stem/progenitor cells make distinct contributions to bone maintenance and repair
    Authors: EC Jeffery, TLA Mann, JA Pool, Z Zhao, SJ Morrison
    Cell Stem Cell, 2022-10-21;29(11):1547-1561.e6.
    Species: Mouse
    Sample Types: Whole Cells
  6. Extracellular biomolecular free radical formation during injury
    Authors: Madeline R. Hines, Jessica E. Goetz, Piedad C. Gomez-Contreras, Samuel N. Rodman, Suryamin Liman, Elise L. Femino et al.
    Free Radical Biology and Medicine
  7. Intranasal immunization with avian paramyxovirus type 3 expressing SARS-CoV-2 spike protein protects hamsters against SARS-CoV-2
    Authors: Hong-Su Park, Yumiko Matsuoka, Cindy Luongo, Lijuan Yang, Celia Santos, Xueqiao Liu et al.
    npj Vaccines
  8. CRISPR activation screen identifies BCL-2 proteins and B3GNT2 as drivers of cancer resistance to T cell-mediated cytotoxicity
    Authors: J Joung, PC Kirchgatte, A Singh, JH Cho, SP Nety, RC Larson, RK Macrae, R Deasy, YY Tseng, MV Maus, F Zhang
    Nature Communications, 2022-03-25;13(1):1606.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation
  9. PAI‐1 induction during kidney injury promotes fibrotic epithelial dysfunction via deregulation of klotho, p53, and TGF‐ beta 1‐receptor signaling
    Authors: Cody C. Gifford, Fei Lian, Jiaqi Tang, Angelica Costello, Roel Goldschmeding, Rohan Samarakoon et al.
    The FASEB Journal
  10. Pluripotent stem cell-derived endometrial stromal fibroblasts in a cyclic, hormone-responsive, coculture model of human decidua
    Authors: VC Cheung, CY Peng, M Marini?, NJ Sakabe, I Aneas, VJ Lynch, C Ober, MA Nobrega, JA Kessler
    Cell Reports, 2021-05-18;35(7):109138.
    Species: Human
    Sample Types: Reference Standard
  11. Oligodendrocyte precursor cell specification is regulated by bidirectional neural progenitor-endothelial cell crosstalk
    Authors: I Paredes, JR Vieira, B Shah, CF Ramunno, J Dyckow, H Adler, M Richter, G Schermann, E Giannakour, L Schirmer, HG Augustin, C Ruiz de Al
    Nature Neuroscience, 2021-01-28;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  12. Cell Adhesion Molecule 1 Contributes to Cell Survival in Crowded Epithelial Monolayers
    Authors: M Hagiyama, R Kimura, A Yoneshige, T Inoue, T Otani, A Ito
    Int J Mol Sci, 2020-06-09;21(11):.
    Species: Human, Rabbit, Rat
    Sample Types: Transfected Whole Cells, Whole Cells
    Applications: Neutralization Control
  13. The C5a/C5aR2 axis promotes renal inflammation and tissue damage
    Authors: T Zhang, KY Wu, N Ma, LL Wei, M Garstka, W Zhou, K Li
    JCI Insight, 2020-04-09;5(7):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  14. On the Role IL-4/IL-13 Heteroreceptor Plays in Regulation of Type 1 Diabetes
    Authors: TK Ukah, AN Cattin-Roy, W Chen, MM Miller, S Barik, H Zaghouani
    J. Immunol., 2017-06-23;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Control
  15. Evidence for chemokine synergy during neutrophil migration in ARDS
    Thorax, 2016-08-05;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  16. Low-dose irradiation affects expression of inflammatory markers in the heart of ApoE -/- mice.
    Authors: Mathias D, Mitchel R, Barclay M, Wyatt H, Bugden M, Priest N, Whitman S, Scholz M, Hildebrandt G, Kamprad M, Glasow A
    PLoS ONE, 2015-03-23;10(3):e0119661.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Control
  17. Neutrophils increase or reduce parasite burden in Trypanosoma cruzi-infected macrophages, depending on host strain: role of neutrophil elastase.
    Authors: Luna-Gomes T, Filardy A, Rocha J, Decote-Ricardo D, LaRocque-de-Freitas I, Morrot A, Bozza P, Castro-Faria-Neto H, DosReis G, Nunes M, Freire-de-Lima C
    PLoS ONE, 2014-03-05;9(3):e90582.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  18. Adipose tissue derived stem cells differentiate into carcinoma-associated fibroblast-like cells under the influence of tumor derived factors
    Authors: Constantin Jotzu, Eckhard Alt, Gabriel Welte, Jie Li, Bryan T. Hennessy, Eswaran Devarajan et al.
    Cell Oncol (Dordr)
  19. PACAP decides neuronal laminar fate via PKA signaling in the developing cerebral cortex.
    Authors: Ohtsuka M, Fukumitsu H, Furukawa S
    Biochem. Biophys. Res. Commun., 2008-03-17;369(4):1144-9.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Control
  20. Characterization of in vitro antimurine thymocyte globulin-induced regulatory T cells that inhibit graft-versus-host disease in vivo.
    Authors: Ruzek MC, Waire JS, Hopkins D, Lacorcia G, Sullivan J, Roberts BL, Richards SM, Nahill SR, Williams JM, Scaria A, Dzuris J, Shankara S, Garman RD
    Blood, 2007-11-19;111(3):1726-34.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  21. CD4+CD25+ regulatory T cells inhibit natural killer cell functions in a transforming growth factor-beta-dependent manner
    Authors: François Ghiringhelli, Cédric Ménard, Magali Terme, Caroline Flament, Julien Taieb, Nathalie Chaput et al.
    The Journal of Experimental Medicine
  22. Astrocytes and retrograde degeneration of nigrostriatal dopaminergic neurons in Parkinson's disease: removing axonal debris
    Authors: Morales I, Puertas-Avendano R, Sanchez A Et al.
    Translational neurodegeneration
  23. Striatal astrocytes engulf dopaminergic debris in Parkinson's disease: A study in an animal model.
    Authors: Morales Ingrid, Sanchez Alberto, Rodriguez-Sabate Clara, Rodriguez Manuel
    PloS One

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