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Normal Mouse IgM Alexa Fluor® 488-conjugated Control

Catalog #: IC015G
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IC015G
R&D Systems Antibodies
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Normal Mouse IgM Alexa Fluor® 488-conjugated Control Summary

Specificity
Serum was obtained from naive (non-immunized) mice and purified for use as a mouse IgM control for flow cytometry applications.
Source
Polyclonal Mouse IgM
Purification
Protein A or G purified
Immunogen
NA
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 488 (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration
Sample
Control
Negative control for use in conjunction with R&D Systems antibodies. This isotype control has been derivatized with a quantity of fluorochrome that matched the F/P (fluorochrome/protein) ratio of R&D Systems monoclonal reagents. The recommended concentration is 5 μL/106 cells.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IgM

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin M
Alternate Names
AGM1; DKFZp686I15196; DKFZp686I15212; FLJ00385; IgM; immunoglobulin heavy constant mu; MGC104996; MGC52291; MU; VH

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Citation for Normal Mouse IgM Alexa Fluor® 488-conjugated Control

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. A Multi-Color Flow Cytometric Assay for Quantifying Dinutuximab Binding to Neuroblastoma Cells in Tumor, Bone Marrow, and Blood
    Authors: Keyel, ME;Furr, KL;Kang, MH;Reynolds, CP;
    Journal of clinical medicine
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

FAQs

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