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Normal Mouse IgM PE-conjugated Control

Catalog #: IC015P
2 Citations Datasheet / COA / SDS

Discontinued Product

IC015P has been discontinued.
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Normal Mouse IgM PE-conjugated Control Summary

Specificity
Serum was obtained from naive (non-immunized) mice and purified for use as a mouse IgM control for flow cytometry applications.
Source
Polyclonal Mouse IgM
Purification
Protein A or G purified
Immunogen
NA
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Control
Negative control for use in conjunction with R&D Systems antibodies. This isotype control has been derivatized with a quantity of fluorochrome that matched the F/P (fluorochrome/protein) ratio of R&D Systems monoclonal reagents. The recommended concentration is 10 μL/106 cells.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IgM

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin M
Alternate Names
AGM1; DKFZp686I15196; DKFZp686I15212; FLJ00385; IgM; immunoglobulin heavy constant mu; MGC104996; MGC52291; MU; VH

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Citations for Normal Mouse IgM PE-conjugated Control

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Deletion of the Sodium-Dependent Glutamate Transporter GLT-1 in Maturing Oligodendrocytes Attenuates Myelination of Callosal Axons During a Postnatal Phase of Central Nervous System Development
    Authors: EJ Thomason, E Suárez-Poz, FS Afshari, PA Rosenberg, JL Dupree, B Fuss
    Oncogene, 2022-06-03;16(0):905299.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry Control
  2. Human primordial germ cell commitment in vitro associates with a unique PRDM14 expression profile.
    Authors: Sugawa F, Arauzo-Bravo M, Yoon J, Kim K, Aramaki S, Wu G, Stehling M, Psathaki O, Hubner K, Scholer H
    EMBO J, 2015-03-06;34(8):1009-24.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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