Normal Rabbit IgG Alexa Fluor® 488-conjugated Control

Name: Normal Rabbit IgG Alexa Fluor® 488-conjugated Control
Brand: R&D Systems
SKU: IC105G

Catalog #: IC105G Datasheet / COA / SDS

Newer Version Available: IC1051G

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Discontinued Product

IC105G has been discontinued and is replaced by IC1051G.

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Normal Rabbit IgG Alexa Fluor® 488-conjugated Control Summary

Specificity

Serum was obtained from naive (non-immunized) rabbits.

Source

Polyclonal Rabbit IgG

Purification

Protein A or G purified

Immunogen

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Alexa Fluor 488 (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration

Sample

Control

Negative control for use in conjunction with R&D Systems antibodies. This isotype control has been derivatized with a quantity of fluorochrome that matched the F/P (fluorochrome/protein) ratio of R&D Systems specific polyclonal antibodies. The recommended concentration is 5 μL/10⁶ cells.

This reagent has not been tested for immunohistochemistry.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name

Immunoglobulin G

Alternate Names

IgG; Immunoglobulin G; ImmunoglobulinG