NucA nuclease Antibody

Catalog #: MAB100635 Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB100635-100
MAB100635-SP

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Summary Data Examples Preparation and Storage Reconstitution Calculator Product Datasheets Related Research Areas

NucA nuclease Antibody Summary

Specificity

Detects NucA nuclease in direct ELISAs.

Source

Monoclonal Mouse IgG₁ Clone # 1028937

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived NucA nuclease
Met1-Asn266
Accession # P13717

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

ELISA

This antibody functions as an ELISA capture antibody when paired with Mouse Anti-NucA nuclease Monoclonal Antibody (Catalog # MAB100634).

This product is intended for assay development on various assay platforms requiring antibody pairs.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

ELISA

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NucA nuclease ELISA Standard Curve. Recombinant NucA nuclease protein was serially diluted 2-fold and captured by Mouse Anti-NucA nuclease Monoclonal Antibody (Catalog # MAB100635) coated on a Clear Polystyrene Microplate (DY990). Mouse Anti-NucA nuclease Monoclonal Antibody (MAB100634) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.