PARP/Apoptosis Chemiluminescent Assay Kit
PARP/Apoptosis Chemiluminescent Assay Kit Summary
Ideal for measuring the in vitro activity of PARP-1 in cell extracts before and during apoptosis, screening of candidate PARP-1 inhibitors and determination of IC50 values.Key Benefits
• Chemiluminescent readout
• 96 well format
• Highly sensitive – detects 0.1 mU PARP ~500 cells
• Dynamic range between 0.1 to 10 mU PARP
• Requires 10-100 ng extract for detection
• Assay Time ~3 hrs
Why Use the PARP/Apoptosis Chemiluminescent Assay Kit?
This ELISA based assay detects poly (ADP-ribose) deposited by PARP-1 onto immobilized histones in a 96-well format. An anti-PAR monoclonal detecting antibody followed by addition of a goat anti-rabbit IgG-HRP secondary and a chemiluminescent HRP substrate yields relative light units (RLU) that correlates with PARP-1 activity. During apoptosis PARP-1 is cleaved with a resulting drop in activity. Etoposide is included as a control apoptosis inducer.
Product Specifications
- Measure PARP-1 activity in cell extracts before and after apoptosis.
- For the screening of candidate PARP-1 inhibitors and determination of IC50 values.
Kit Contents
• 10X Activated DNA
• PARP-HSA, 10 mUnits/µl
• 20 mM NAD
• Anti-PAR monoclonal Antibody
• Goat anti-mouse IgG-HRP
• 10 mM Etoposide
• 20X I-PAR Assay Buffer
• PARP PeroxyGlow A
• PARP PeroxyGlow™ B
• 5X Antibody Diluent
• Histone-Coated White Strip Well Plate, I-PAR
Specifications
Limitations
For research use only. Not for diagnostic use.
Product Datasheets
Citation for PARP/Apoptosis Chemiluminescent Assay Kit
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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MUC1-C INTEGRATES CHROMATIN REMODELING AND PARP1 ACTIVITY IN THE DNA DAMAGE RESPONSE OF TRIPLE-NEGATIVE BREAST CANCER CELLS
Authors: M Yamamoto, C Jin, T Hata, Y Yasumizu, Y Zhang, D Hong, T Maeda, M Miyo, M Hiraki, Y Suzuki, K Hinohara, H Rajabi, D Kufe
Cancer Res., 2019-03-01;0(0):. 2019-03-01
FAQs
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Is it possible to purchase a smaller version of the PARP/Apoptosis Chemiluminescent Assay Kit?
R&D Systems does not offer a smaller version of this kit. However, plates are provided in stripwell format, so the user is able to test fewer wells and save the remaining plate strips for later use.
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Is it possible to test partial plates when using the PARP/Apoptosis Chemiluminescent Assay Kit?
The histone-coated plates are provided in stripwell format. To run partial plates, remove excess microplate strips, seal in the provided pouch, and store at 2-8 °C with a dessicant.
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The PARP/Apoptosis Chemiluminescent Assay was used to assess signs of apoptotic induction by Temozolomide in Temozolomide-Resistant versus parental glioma lines. Results were consistent.
