Pepstatin A Janelia Fluor® 526
Chemical Name: 2-(3-(3,3-Difluoroazetidin-1-ium-1-ylidene)-6-(3,3-difluoroazetidin-1-yl)-2,7-difluoro-3H-xanthen-9-yl)-4-(((6S,9S,12S,13S,17S,20S,21S)-13,21-dihydroxy-12,20-diisobutyl-6,9-diisopropyl-2,17-dimethyl-4,7,10,15,18,23-hexaoxo-5,8,11,16,19,24-hexaazatriacontan-30-yl)carbamoyl)benzoate trifluoroacetate
Purity: ≥90%
Biological Activity
Pepstatin A Janelia Fluor® 526 is a fluorogenic green-emitting lysosome tracker and stain. No wash step is required when used for multicolor microscopy experiments. Pepstatin A Janelia Fluor® 526 can be used for live cell imaging, in confocal microscopy and super-resolution microscopy techniques including 3D-SIM (structured illumination microscopy) and STED, as well as two-color lattice light-sheet microscopy (lls). Excitation maximum = 530 nm, emission maximum = 549 nm.To measure the absorbance spectrum of this dye we recommend the following solvent: TFE plus 0.1% TFA.
Scientific Data
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Application of Pepstatin A Janelia Fluor® 526 in live U2OS cells. Live U2OS cells were stained with Pepstatin A Janelia Fluor® 526 (2 µM) for 1 hour with Verapamil (10 µM) and imaged without an intermediate washing step on a LSM980 confocal microscope (Zeiss) using the following configuration: excitation: 514 nm / emission: 526–615 nm. Image kindly provided by Prof. Luke Lavis, Howard Hughes Medical Institute, Janelia Research Campus.
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Application of Pepstatin A Janelia Fluor® 526 in fixed U2OS cells. Fixed U2OS cells were stained with Pepstatin A Janelia Fluor® 526 (2 µM) for 1 hour in 0.1 M sodium acetate buffer pH 4.5. Cells were imaged on a LSM980 confocal microscope (Zeiss) using the following configuration: excitation: 514 nm / emission: 526–615 nm. Image kindly provided by Prof. Luke Lavis, Howard Hughes Medical Institute, Janelia Research Campus
Optical Data for Pepstatin A Janelia Fluor® 526
| Emission Color | Yellow |
|---|---|
| λabs | 530 nm |
| λem | 549 nm |
| Extinction Coefficient (ε) | 118,000 M-1cm-1 |
| Quantum Yield (φ) | 0.87 |
| Application | Confocal Microscopy, STED, 3D-SIM, Lattice Light-sheet Microscopy |
| Alternative For | Lysotracker™- Lysotracker is a registered trademark of Thermo Fisher |
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectral Viewer
Technical Data
The technical data provided above is for guidance only.
For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Additional Information
Background References
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Rational design of fluorogenic and spontaneously blinking labels for super-resolution imaging.
Zheng et al.
ACS Cent.Sci., 2019;5:1602
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