Home / GAP-43 / Phospho-GAP‑43 (S41) Antibody

Phospho-GAP‑43 (S41) Antibody

Catalog #: PPS006
1 Citation Datasheet / COA / SDS
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PPS006
Detection of Phospho-GAP‑43 (S41) by Western blot.
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Phospho-GAP‑43 (S41) Antibody Summary

Species Reactivity
Human, Mouse, Rat, Bovine, Canine, Chicken, Primate, Xenopus, Zebrafish
Specificity
Specific for the ~50 kDa GAP-43 protein phosphorylated at S41 in Western blots. In some tissues the antibody also recognizes a higher molecular weight protein, that is also recognized by the Pan GAP-43 antibody. This may be a GAP-43 aggregate or oligomer.
Source
Polyclonal Rabbit IgG
Purification
Antigen Affinity-purified
Immunogen
Phosphopeptide corresponding to amino acid residues surrounding the phospho-S41 of GAP-43
Formulation
100 μL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50% glycerol.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1:1000 dilution
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Phospho-GAP-43 (S41) antibody by Western Blot. View Larger

Detection of Phospho-GAP‑43 (S41) by Western blot. Western blot of rat cortex lysate showing specific immunolabeling of the ~50 kDa GAP-43 protein phosphorylated at S41 (Control). The phosphospecificity of this labeling is demonstrated by treatment with 1200 U of lambda Phosphatase ( lambda -PPase) for 30 minutes before being exposed to the Anti-Phospho-GAP-43 (S41). The immunolabeling is completely eliminated by treatment with lambda -PPase.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
For long-term storage, ≤ -20° C is recommended. Product is stable at ≤ -20° C for at least 1 year.

Background: GAP-43

GAP-43 (Growth Associated Protein 43 kDa), also known as Neuromodulin, is a 24 kDa, neuron growth cone-associated member of the neuromodulin family. Apparently due to its tertiary structure, it runs anomalously at 43 kDa on SDS-polyacrylamide gel. Rat GAP-43 is a 226 amino acid (aa) protein that contains an N-terminal lipid binding motif, an 11 aa IQ domain (IQ:Ile-Gln-X-Ser-etc) (aa 38 - 48), and a C-terminus with four potential serine phosphorylation sites. The molecule may utilize the IQ domain to bind calmodulin. Protein kinase C-mediated phosphorylation of the Serine at 41 within this site inhibits this interaction. Unphosphorylated, calmodulin-bound GAP-43 is always associated with neurite retraction, while S41 phosphorylated GAP-43 stabilizes actin filaments and promotes growth cone extension.

Long Name
Growth Associated Protein 43
Entrez Gene IDs
2596 (Human); 14432 (Mouse); 29423 (Rat)
Alternate Names
Axonal membrane protein GAP-43; B-50; Basp2; calmodulin-binding protein P-57; GAP43; GAP-43; growth associated protein 43; Growth-associated protein 43; nerve growth-related peptide GAP43; Neural phosphoprotein B-50; Neuromodulin; neuron growth-associated protein 43; PP46; protein F1

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Citation for Phospho-GAP‑43 (S41) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Adipose mTORC2 is essential for sensory innervation in white adipose tissue and whole-body energy homeostasis
    Authors: IC Frei, D Weissenber, D Ritz, W Heusermann, M Colombi, M Shimobayas, MN Hall
    Molecular Metabolism, 2022-08-23;0(0):101580.
    Species: Mouse
    Sample Types: Tissue Lysates
    Applications: Western Blot

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