Phospho-GluR1 (S831) Antibody

Catalog #: PPS007
1 Citation Datasheet / COA / SDS

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PPS007

Detection of Phospho-GluR1 (S831) by Western Blot.

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All GluR1 Antibodies

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Phospho-GluR1 (S831) Antibody Summary

Species Reactivity

Human, Mouse, Rat, Canine, Chicken

Specificity

Specific for the ~100 kDa GluR1 protein phosphorylated at S831 in Western blots

Source

Polyclonal Rabbit IgG

Purification

Antigen Affinity-purified

Immunogen

Phosphopeptide corresponding to amino acid residues surrounding the phospho-S831 of GluR1

Formulation

150 μL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50% glycerol.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1:1000 dilution

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Phospho-GluR1 (S831) by Western Blot. Western blot of rat hippocampal lysate showing specific immunolabeling of the ~100 kDa GluR1 protein phosphorylated at S831 (Control). The phosphospecificity of this labeling is demonstrated by treatment with 1200 U of lambda Phosphatase ( lambda -PPase) for 30 minutes before being exposed to the Anti-Phospho-GluR1 (S831). The immunolabeling is completely eliminated by treatment with lambda -PPase.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

For long-term storage, ≤ -20° C is recommended. Product is stable at ≤ -20° C for at least 1 year.

Background: GluR1

Rat GluR1 is a 907 amino acid, 4-transmembrane protein that belongs to the glutamate-gated ion channel family. It is one of four AMPA receptor subunits that form a functional heterotetrameric glutamate receptor. GluR1 only interacts with GluR2. GluR1 has two key serine residues in the C-terminal extracellular region. Serine 831 is constitutively unphosphorylated. Upon exposure to a neurotransmitter, it is phosphorylated by CaMKII leading to a potentiation of glutamate-mediated current. Serine 845 is constitutively phosphorylated by Protein Kinase A (PKA). The presence of the phosphate insures membrane localization of the subunit. Following NMDA receptor activation, S845 is dephosphorylated with subsequent subunit internalization.

Long Name

Glutamate Receptor 1

Entrez Gene IDs

2890 (Human); 14799 (Mouse); 50592 (Rat)

Alternate Names

AMPA 1; AMPA-selective glutamate receptor 1; GluA1; GLUH1; GluR1; gluR-1; GLUR1gluR-A; GLURA; GluR-A; gluR-K1; glutamate receptor 1; Glutamate receptor ionotropic, AMPA 1; glutamate receptor, ionotropic, AMPA 1; GRIA1; HBGR1; MGC133252

Product Datasheets

Product Datasheet

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Citation for Phospho-GluR1 (S831) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Endosomal Dysfunction Induced by Directly Overactivating Rab5 Recapitulates Prodromal and Neurodegenerative Features of Alzheimer's Disease
Authors: A Pensalfini, S Kim, S Subbanna, C Bleiwas, CN Goulbourne, PH Stavrides, Y Jiang, JH Lee, S Darji, M Pawlik, C Huo, J Peddy, MJ Berg, JF Smiley, BS Basavaraja, RA Nixon
Cell Rep, 2020-11-24;33(8):108420.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot