Phospho-PLKK (S482/486/490) Antibody

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Background: PLKK

Xenopus Polo-Like Kinase Kinase (xPLKK1) is a 120 kDa Ser/Thr kinase that is associated with 67 kDa PLx1 (Polo-Like Xenopus Kinase 1). PLx1 is involved with spindle assembly and centrosome maturation during the late G2 and M cell cycle phase. A cell’s entry into mitosis is controlled by the activation of Cyclin B/cdc2. cdc2 is constitutively phosphorylated and inactive, and activated by dephosphorylation of Tyr at position 15. The phosphatase that accomplishes this is cdc25C, which is activated by phosphorylation at multiple sites by a polo-like kinase termed PLx1 (PLK1 in mammals). The activating enzyme that acts on PLx1 is under investigation, and the kinase termed xPLKK1 has been identified as a candidate. xPLKK1 is 950 amino acids (aa) in size and contains a 264 amino acid kinase domain (aa 31 - 294). It contains three serines within a nine amino acid span (S482/486/490) which, when phosphorylated, activate the enzyme. Activation of PLx1 appears to depend on the phosphorylation of T201. And while xPLKK1 phosphorylates PLx1, it does so only on T10. Thus, its role in PLx1 activation is unclear. Notably, PLx1 has been shown to activate xPLKK1 on S482/486/490, suggesting that xPLKK1 may actually be a downstream target of PLx1 rather than an activator of PLx1.

Product Datasheets

Related Research Areas

• Checkpoint
• Intracellular Kinases
• Intracellular Kinases in the Akt Pathway
• Search Kinases and Phosphatases