Phospho-STAT2 (Y690) Alexa Fluor® 488‑conjugated Antibody

Catalog #: IC8627G Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
IC8627G-025

Detection of Phospho-STAT2 (Y690) in Daudi Human Cell Line by Flow Cytometry.

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Phospho-STAT2 (Y690) Alexa Fluor® 488‑conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human phospho-STAT2 (Y690) in Western blots.

Source

Recombinant Monoclonal Rabbit IgG Clone # 1021D

Purification

Protein A or G purified from cell culture supernatant

Immunogen

Phosphopeptide containing the human STAT2 Y690 site

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Alexa Fluor 488 (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration

Sample

Intracellular Staining by Flow Cytometry

5 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry

Detection of Phospho-STAT2 (Y689) antibody in Daudi Human Cell Line antibody by Flow Cytometry.

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Detection of Phospho-STAT2 (Y690) in Daudi Human Cell Line by Flow Cytometry. Daudi human Burkitt's lymphoma cells were serum starved ovenight, then left untreated (open histogram) or treated with 500 U/mL Recombinant Human IFN-aA (11100-1) for 20 minutes was stained with Rabbit Anti-Human STAT2 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # IC8627G, filled histogram) or isotype control antibody (IC105G, not shown). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with 90% methanol. View our protocol for Staining Intracellular Molecules.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: STAT2

STAT2 (signal transducer and activator of transcription 2) is a 113 kDa member of the STAT family of cytoplasmic transcription factors. STAT members generally mediate cytokine, growth factor and hormone receptor signal transduction. STAT2 is associated with type I ( alpha - and beta -) interferon signaling. All STATs contain an N‑terminal oligomerization domain, a DNA-binding domain, and an SH2-association region. STAT2 is phosphorylated at Y690 by receptor-associated Janus kinases (JAKs) leading to STAT2 dimerization and subsequent translocation to the nucleus to activate gene transcription.

Long Name

Signal Transducer and Activator of Transcription 2

Entrez Gene IDs

6773 (Human); 20847 (Mouse)

Alternate Names

interferon alpha induced transcriptional activator; ISGF-3; MGC59816; P113; signal transducer and activator of transcription 2; signal transducer and activator of transcription 2, 113kD; signal transducer and activator of transcription 2, 113kDa; STAT113; STAT2