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Phospho-Tyrosine HRP-conjugated Antibody

Catalog #: HAM1676
10 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
HAM1676
Detection of Phospho-Tyrosine by Western Blot.
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Product Details
Citations (10)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Phospho-Tyrosine HRP-conjugated Antibody Summary

Specificity
Detects endogenous proteins containing phosphorylated tyrosine residues. ELISA and 2D Western blot analyses using pervanadate-treated cell lysates indicate that it binds Phospho-Tyrosine in a broad manner largely independent of the surrounding amino acid sequence. Does not cross-react with proteins or peptides containing phosphorylated serine or threonine residues.
Source
Monoclonal Mouse IgG1 Clone # 179003
Formulation
Supplied as a 0.2 μm filtered solution in phosphate-buffered saline (PBS) with stabilizers and preservatives.
Label
HRP(Excitation= nm, Emission= nm)

Applications

Recommended Concentration
Sample
Western Blot
Please refer to the PDF datasheet for specifications.
ELISA
Please refer to the PDF datasheet for specifications.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Phospho-Tyrosine antibody by Western Blot. View Larger

Detection of Phospho-Tyrosine by Western Blot.

ELISA View Larger

Phospho-PDGF R beta ELISA Using HAM1676 as Detection Antibody.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
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Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Phospho-Tyrosine

Phosphorylation of tyrosine residues in signaling proteins by protein tyrosine kinases mediates a variety of cellular processes, including cell growth, differentiation, adhesion, motility, death, and metabolism. Dysregulation of tyrosine phosphorylation has been implicated in the development of many human diseases, such as diabetes and cancer. Antibodies specific for phospho-tyrosine have been invaluable reagents in the studies of signaling pathways initiated by tyrosine phosphorylation.

Alternate Names
2 amino 3(4 hydroxyphenyl) propanoic acid; 4 hydroxyphenylalanine; PhosphoTyrosine; pTyrosine; pY20; Tyrosine

Product Datasheets

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Citations for Phospho-Tyrosine HRP-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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  1. PDGF-PDGFR network differentially regulates the fate, migration, proliferation, and cell cycle progression of myogenic cells
    Authors: O Contreras, A Córdova-Ca, E Brandan
    Cellular Signalling, 2021-05-08;84(0):110036.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Siglec-9 regulates an effector memory CD8+ T-cell subset that congregates in the melanoma tumor microenvironment
    Authors: Q Haas, K Frias Boli, C Jandus, C Schneider, C Simillion, MA Stanczak, M Haubitz, SM Seyed Jafa, A Zippelius, GM Baerlocher, H Läubli, RE Hunger, P Romero, HU Simon, S von Gunten
    Cancer Immunol Res, 2019-04-15;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: ELISA Development (Detection)
  3. Epidermal Growth Factor Receptor Extracellular Domain Mutations in Glioblastoma Present Opportunities for Clinical Imaging and Therapeutic Development
    Authors: Zev A. Binder, Amy Haseley Thorne, Spyridon Bakas, E. Paul Wileyto, Michel Bilello, Hamed Akbari et al.
    Cancer Cell
  4. Tough Composite Hydrogels with High Loading and Local Release of Biological Drugs
    Authors: J Li, E Weber, S Guth-Gunde, M Schuleit, A Kuttler, C Halleux, N Accart, A Doelemeyer, A Basler, B Tigani, K Wuersch, M Fornaro, M Kneissel, A Stafford, BR Freedman, DJ Mooney
    Adv Healthc Mater, 2018-02-14;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: ELISA
  5. Interaction of cytokeratin 19 head domain and HER2 in the cytoplasm leads to activation of HER2-Erk pathway
    Sci Rep, 2016-12-23;6(0):39557.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. Claudin-4 controls the receptor tyrosine kinase EphA2 pro-oncogenic switch through ss-catenin.
    Authors: Shang X, Lin X, Howell S
    Cell Commun Signal, 2014-10-25;12(1):59.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  7. Increased membrane cholesterol in lymphocytes diverts T-cells toward an inflammatory response.
    Authors: Surls J, Nazarov-Stoica C, Kehl M, Olsen C, Casares S, Brumeanu TD
    PLoS ONE, 2012-06-19;7(6):e38733.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  8. The angiotensin IV analog Nle-Tyr-Leu-psi-(CH2-NH2)3-4-His-Pro-Phe (norleual) can act as a hepatocyte growth factor/c-Met inhibitor
    Authors: B. J. Yamamoto, P. D. Elias, J. A. Masino, B. D. Hudson, A. T. McCoy, Z. J. Anderson et al.
    Journal of Pharmacology and Experimental Therapeutics
  9. Human epidermal growth factor receptor (HER-1:HER-3) Fc-mediated heterodimer has broad antiproliferative activity in vitro and in human tumor xenografts.
    Authors: Sarup J, Jin P, Turin L, Bai X, Beryt M, Brdlik C, Higaki JN, Jorgensen B, Lau FW, Lindley P, Liu J, Ni I, Rozzelle J, Kumari R, Watson SA, Zhang J, Shepard HM
    Mol. Cancer Ther., 2008-10-01;7(10):3223-36.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  10. Cupredoxin-cancer interrelationship: azurin binding with EphB2, interference in EphB2 tyrosine phosphorylation, and inhibition of cancer growth.
    Authors: Chaudhari A, Mahfouz M, Fialho AM, Yamada T, Granja AT, Zhu Y, Hashimoto W, Schlarb-Ridley B, Cho W, Das Gupta TK, Chakrabarty AM
    Biochemistry, 2007-01-24;46(7):1799-810.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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