Polybrene

Key Product Details

Product Description

Polybrene is a cationic polymer that improves lentiviral and adenoviral transduction efficiency in mammalian cells in vitro. It also enhances DNA transfection in many types of cells. It has a neutralizing effect on negatively charged DNA and viral particles which facilitates their attachment and entry into host cells. Polybrene is suitable for use with CRISPR/Cas9 protocols. It is commonly used in combination with Protamine sulfate (Cat. No. 8822) to enhance the delivery of adeno-associated virus (AAV) and lentiviral vectors.

Polybrene is a lyophilized solid and can be prepared as a stock solution by adding 1 mL of sterile ultra-pure water directly to the vial (final stock concentration 10 mg/mL).

For further information view the product protocol.

Product Specifications for Polybrene

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Calculators

Background References

References are publications that support the biological activity of the product. See our Citations tab to view 22 publications citing the usage of this product.

• Elegheert Lentiviral transduction of mammalian cells for fast, scalable and high-level production of soluble and membrane proteins. Nat.Protoc. 2018 PMID: 30455477
• Kumar Generation and validation of CRISPR-engineered human natural killer cell lines for research and therapeutic applications. STAR Protoc 2021 PMID: 34746857
• Joung CRISPR activation screen identifies BCL-2 proteins and B3GNT2 as drivers of cancer resistance to T cell-mediated cytotoxicity. Nat.Commun 2022 PMID: 35338135
• Navarro-Guerrero Genome-wide CRISPR/Cas9-knockout in human induced Pluripotent Stem Cell (iPSC)-derived macrophages. Sci.Rep. 2021 PMID: 33608581
• Strack Breaking entry-and species barriers: LentiBOOST ® Plus polybrene enhances transduction efficacy of dendritic cells and monocytes by adenovirus 5. Viruses 2022 PMID: 35062296
• Zhao Adenovirus-mediated gene transfer in mesenchymal stem cells can be significantly enhanced by the cationic polymer polybrene. PLoS One 2014 PMID: 24658746
• Bolis Dynamic prostate cancer transcriptome analysis delineates the trajectory to disease progression. Nat.Commun. 2021 PMID: 34857732
• Shayakhmetov Dependence of adenovirus infectivity on length of the fiber shaft domain. J.Virol. 2000 PMID: 11044071
• Abe Polybrene increases the efficiency of gene transfer by lipofection. Gene Ther. 1998 PMID: 9797877
• Davis Polybrene increases retrovirus gene transfer efficiency by enhancing receptor-independent virus adsorption on target cell membranes. Biophys.Chem. 2002 PMID: 12050007

Product Documents for Polybrene