Polybrene

Biological Activity

Polybrene is a cationic polymer that improves lentiviral and adenoviral transduction efficiency in mammalian cells in vitro. It also enhances DNA transfection in many types of cells. It has a neutralizing effect on negatively charged DNA and viral particles which facilitates their attachment and entry into host cells. Polybrene is suitable for use with CRISPR/Cas9 protocols.

Polybrene is a lyophilized solid and can be prepared as a stock solution by adding 1 mL of sterile ultra-pure water directly to the vial (final stock concentration 10 mg/mL).

For further information view the product protocol.

Technical Data

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

Product Datasheets